ND2 plugin issue

[Jinghui]

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[Jinghui]

Dear ImageJ users,

I have a question about quantifying ND2 images in Image J. I have ND2 plugin
in image J. I want to create a Macro which allows me to open ND2 file
containing 3channels, set a threshold in each channel, measure
threshold-area in each channel, and export the measure results as an excel
file. I record a macro and try to run it, but it always tells me there is no
image open. Do you know if this is because I use ND2 plugin or I did
something wrong? Thanks a lot for your help.
 
Jinghui
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[Jinghui]

Dear ImageJ users,

I have a question about quantifying ND2 images in Image J. I have ND2 plugin in image J. I want to create a Macro which allows me to open ND2 file containing 3channels, set a threshold in each channel, measure threshold-area in each channel, and export the measure results as an excel file. I record a macro and try to run it, but it always tells me there is no image open. Do you know if this is because I use ND2 plugin or I did something wrong? Thanks a lot for your help.
 
Jinghui

[ed3203]

Hi,

I would recommend exporting into a multi image tiff file and working from there.

The processes of imagej don't seem to recognise the nd2 file, much simpler to export the rgb image into individual stacks.

Let us know how you get on and perhaps even post your macro

ed

[Cammer, Michael]

The LOCI plugins handle the nd2 file format fine in ImageJ on the PCs and Macs in our lab.
Cheers,
Michael

_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

________________________________________
From: ImageJ Interest Group [[hidden email]] On Behalf Of ed3203 [[hidden email]]
Sent: Wednesday, February 02, 2011 5:48 AM
To: [hidden email]
Subject: Re: ND2 plugin issue

Hi,

I would recommend exporting into a multi image tiff file and working from
there.

The processes of imagej don't seem to recognise the nd2 file, much simpler
to export the rgb image into individual stacks.

Let us know how you get on and perhaps even post your macro

ed
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View this message in context: http://imagej.588099.n2.nabble.com/ND2-plugin-issue-tp5963817p5984270.html
Sent from the ImageJ mailing list archive at Nabble.com.

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[Jinghui]

Hi All,

Thanks for all suggestions. I have downloaded LOCI plugin and it processes ND2 file pretty well. Thanks a lot for help.

Jinghui 

On Wed, Feb 2, 2011 at 6:16 AM, Cammer, Michael [via ImageJ] <[hidden email]> wrote:

The LOCI plugins handle the nd2 file format fine in ImageJ on the PCs and Macs in our lab.
Cheers,
Michael

_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

________________________________________
From: ImageJ Interest Group [[hidden email]] On Behalf Of ed3203 [[hidden email]]
Sent: Wednesday, February 02, 2011 5:48 AM
To: [hidden email]
Subject: Re: ND2 plugin issue

Hi,

I would recommend exporting into a multi image tiff file and working from
there.

The processes of imagej don't seem to recognise the nd2 file, much simpler
to export the rgb image into individual stacks.

Let us know how you get on and perhaps even post your macro

ed

--
View this message in context: http://imagej.588099.n2.nabble.com/ND2-plugin-issue-tp5963817p5984270.html
Sent from the ImageJ mailing list archive at Nabble.com.

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This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
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