Alejandra,
Do you have an example before and after image you could share?
It's hard to completely understand what you mean by "shift".
Also, since you don't care about accuracy so much as relative difference,
do you need individual fiber measurements or can you get away with a
measurement for lots of fibers at once (i.e. everything visible in your
image)?
B
On Tue, Jan 5, 2016 at 12:57 PM, Alejandra CB <
[hidden email]> wrote:
> Hello,
> I was wondering if the following is possible using image J:
>
> I have pictures of some fibers before and after they have been coated with
> a thick polymer film. The pictures are taken at the same magnification and
> correspond to the same area, however after applying the film the fibers
> tilt a bit so the images don't exactly superimpose. I am interested in
> calculating the areas occupied by the fibers so I can compare the before
> and after coating values.
> I am trying to make a quick assessment of the increase in thickness due to
> the coating so of course I know I could measure fiber by fiber but that is
> not the point since I have to evaluate many coatings and do not need a very
> precise value, it is more for comparison purposes. Any ideas if something
> of this sort can be done?, how?
> I've tried subtracting images but that gives me another image when I need
> a numeric value plus the tilting of the fibers makes it difficult to even
> say anything from the resulting image. I have binarized the images and
> tried to convert one in background or mask to subtract from the "after"
> image but have the same issue with the tilted fibers. Then I ran out of my
> ImageJ knowledge so as you can see it is very limited.
> Any help is really appreciated.
> Alejandra
>
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