Oil red O

[Wim Van Den Broeck]

Dear friends,

I am just starting using Image J, so no experience at all.

However, I do have a very particular question. I need to analyse cryosections stained with Oil Red O, so is there any Oil red O plugin available ?

Thanks a lot for your answers / help.

Kind regards,

Wim Van den Broeck.

[cid:image001.png@01D21591.203AEEA0]






Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell Biology and Histology
T [phone]

Department of Morphology
Faculty of Veterinary Medicine, D5, Salisburylaan 133, 9820 Merelbeke, BELGIUM
T [phone] / F [phone]

www.ugent.be<https://www.ugent.be/>
www.facebook.com/ugent<https://www.facebook.com/ugent>
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[Adrián Villalba]

Dear Prof. Van den Broeck,

Which is exactly what you want to analyze - or the expected output -? If
you want to quantify the number of particles stained in Oil red, then maybe
you can try to Threshold (Adjust-Threshold) and then Analyze-Particle
analizer, playing with the different min/max values in the thresholding.

All my best,

2017-08-29 15:47 GMT+02:00 Wim Van Den Broeck <[hidden email]>:

> Dear friends,
>
> I am just starting using Image J, so no experience at all.
>
> However, I do have a very particular question. I need to analyse
> cryosections stained with Oil Red O, so is there any Oil red O plugin
> available ?
>
> Thanks a lot for your answers / help.
>
> Kind regards,
>
> Wim Van den Broeck.
>
> [cid:image001.png@01D21591.203AEEA0]
>
>
>
>
>
>
> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
> Cell Biology and Histology
> T [phone]
>
> Department of Morphology
> Faculty of Veterinary Medicine, D5, Salisburylaan 133, 9820 Merelbeke,
> BELGIUM
> T [phone] / F [phone]
>
> www.ugent.be<https://www.ugent.be/>
> www.facebook.com/ugent<https://www.facebook.com/ugent>
> www.twitter.com/ugent<https://www.twitter.com/ugent>
>
> e-maildisclaimer<https://helpdesk.ugent.be/e-maildisclaimer.php>
>
>
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

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[Daan VAN DEN BRINK]

Goedemiddag,

I have some experience quantifying lipid stains in biological samples
using ImageJ, and usually calculate %area covered by staining, along the
line of what Adrián suggests. To analyze my samples I have made a script
that does the business. Maybe you could attach a sample image to show us
precisely what you need?

Daan

ps. in my experience Oil Red O fades very quickly which might make
quantification difficult.

On 29/08/17 16:08, Adrián Villalba wrote:

> Dear Prof. Van den Broeck,
>
> Which is exactly what you want to analyze - or the expected output -? If
> you want to quantify the number of particles stained in Oil red, then maybe
> you can try to Threshold (Adjust-Threshold) and then Analyze-Particle
> analizer, playing with the different min/max values in the thresholding.
>
> All my best,
>
> 2017-08-29 15:47 GMT+02:00 Wim Van Den Broeck <[hidden email]>:
>
>> Dear friends,
>>
>> I am just starting using Image J, so no experience at all.
>>
>> However, I do have a very particular question. I need to analyse
>> cryosections stained with Oil Red O, so is there any Oil red O plugin
>> available ?
>>
>> Thanks a lot for your answers / help.
>>
>> Kind regards,
>>
>> Wim Van den Broeck.
>>
>> [cid:image001.png@01D21591.203AEEA0]
>>
>>
>>
>>
>>
>>
>> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
>> Cell Biology and Histology
>> T [phone]
>>
>> Department of Morphology
>> Faculty of Veterinary Medicine, D5, Salisburylaan 133, 9820 Merelbeke,
>> BELGIUM
>> T [phone] / F [phone]
>>
>> www.ugent.be<https://www.ugent.be/>
>> www.facebook.com/ugent<https://www.facebook.com/ugent>
>> www.twitter.com/ugent<https://www.twitter.com/ugent>
>>
>> e-maildisclaimer<https://helpdesk.ugent.be/e-maildisclaimer.php>
>>
>>
>>
>>
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
>

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