Overay on ImageJ

[Juan Francisco-2]

Dear ImageJ users:
I try to do the overlay of one image over another, pls, someone knows hoe to do this??
For insatnce: I take a image and then I do a Find max . I would like to overlay the image resulting of FInd Max operation , onto the original image.
 
Thank you very much for your help
 
JFC

[Reinhard Mayr aka Czerwinski]

JFC,

maybe this tutorial can help you.

Cheers,

Reinhard.

You have 2 16bit grayscale images you want to overlay by assigning each of the source images a distinct color.
close all image windows in ImageJ and open the images to overlay

menu item Image/Stack/Images to Stack: now both images are unified in a stacked image. Use the slider on the bottom of the window to change the active layer (the layer number is reflected in the z coordinate in the main window).

in the channels tool (menu Image/Hyperstacks/Channels Tool), transform the stacked image to a composite image (i.e. each layer represents a color channel) by More>>/Make Composite. Select display mode Composite.
adjust contrast for each channel (use the slider on the bottom of the window to switch the active channel)

if desired, the LUT (look-up-table) for each channel can be set via menu item Image/Color/Edit LUT

when finished colorizing the picture, use Channels Tool/More/Convert to RGB to create a regular RGB image from your composite image.
save file

-------- Original-Nachricht --------
> Datum: Wed, 10 Sep 2008 10:39:59 +0000
> Von: Juan Francisco <[hidden email]>
> An: [hidden email]
> Betreff: Overay on ImageJ

> Dear ImageJ users:
> I try to do the overlay of one image over another, pls, someone knows hoe
> to do this??
> For insatnce: I take a image and then I do a Find max . I would like to
> overlay the image resulting of FInd Max operation , onto the original image.
>  
> Thank you very much for your help
>  
> JFC
>
>
>      

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[Jonathan Jackson-2]

Dear Juan,

If the overlay is just for visualization purposes, you could try the
Concatenate_ plugin.  To get an overlay with Concatenate_, use the option
'4D stack' with the 4th dimension set as 'Channels'.  Once the new stack is
created, change to 'overlay' mode.  The colour and window / levels can be
set independently for each component.

This requires both the Concatenate_ plugin and Image5D to be installed:
http://rsb.info.nih.gov/ij/plugins/concatenate.html
http://rsb.info.nih.gov/ij/plugins/image5d.html

regards,
Jon

On Wed, 10 Sep 2008 10:39:59 +0000, Juan Francisco <[hidden email]> wrote:

>Dear ImageJ users:
>I try to do the overlay of one image over another, pls, someone knows hoe
to do this??
>For insatnce: I take a image and then I do a Find max . I would like to
overlay the image resulting of FInd Max operation , onto the original image.
>Â
>Thank you very much for your help
>Â
>JFC