Overlay of images

[Oliver Bannach]

Hi all,

how do I overlay a red and a green image to obtain a third image with
colocalized pixels shown in yellow?
Any help is appreciated. Thank you.

Cheers
Oliver

[Joachim Wesner]

Hi Oliver,

this should be possible with RGB merge (built-in)!

                                                                           
 Installa This plugin is built into ImageJ 1.29 and later as the          
 tion:    Image/Color/RGB Merge command.                                  
                                                                           
 Descript Combines one, two or three 8-bit grayscale or RGB images or      
 ion:     stacks into one RGB image or stack.                              
                                                                           




ImageJ Interest Group <[hidden email]> schrieb am 21.04.2008 17:08:50:

> Hi all,
>
> how do I overlay a red and a green image to obtain a third image with
> colocalized pixels shown in yellow?
> Any help is appreciated. Thank you.
>
> Cheers
> Oliver


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

[Joel Sheffield]

You can use Image>color>RGB merge.  If you merge the two images, and
any pixel contains equal amounts of r and g, the resultant color will
be yellow.  HOWEVER, you should be aware that colocalization is a
much more complex phenomenon. The archives will reveal quite a
discussion about it.   I suggest that you take a look at the
information provided with the JACOP plugin in the ImageJ plugins
website.

Joel


> Hi all,
>
> how do I overlay a red and a green image to obtain a third image with
> colocalized pixels shown in yellow?
> Any help is appreciated. Thank you.
>
> Cheers
> Oliver


--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
[hidden email]  
[phone], fax [phone]
http://astro.temple.edu/~jbs

[Elena Kardash]

Hi Oliver,
try this one:
Image>Color>RGB merge, then select the respective images,
works nice for me
Ch,elena


On 21 Apr 2008, at 17:08, Oliver Bannach wrote:

> Hi all,
>
> how do I overlay a red and a green image to obtain a third image  
> with colocalized pixels shown in yellow?
> Any help is appreciated. Thank you.
>
> Cheers
> Oliver

-
Elena Kardash
Institute of Cell Biology, ZMBE
Von-Esmarch-Straße 56
D-48149 Münster
phone: [phone]
           [phone]
fax: [phone]

[Moninger, Thomas]

Group,

I have a time series of 128x128 pixel images that I would like to do a
pixel by pixel FFT,  ideally generating a bell curve/power spectrum
showing the frequency of intensity fluctuation. They are transmitted
light images of beating cilia.

Can't seem to figure out how to do this....any ideas?

Thank you,

Tom

Thomas Moninger [hidden email]
University of Iowa Central Microscopy Research Facilities
www.uiowa.edu/~cemrf
P Think Green before printing this e-mail!



Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.

[Gluender]

>Group,
>
>I have a time series of 128x128 pixel images that I would like to do a
>pixel by pixel FFT,  ideally generating a bell curve/power spectrum
>showing the frequency of intensity fluctuation. They are transmitted
>light images of beating cilia.
>
>Can't seem to figure out how to do this....any ideas?
>
>Thank you,
>
>Tom
>
>Thomas Moninger [hidden email]
>University of Iowa Central Microscopy Research Facilities
>www.uiowa.edu/~cemrf

A similar question came up on this list on 23. Oct 2007.

My reply:

what you are looking for is the 1D Fourier Transformation [of a
single pixel as a function of time] which has no direct relation to
image processing. Consequently, you may have a look at general signal
rpocessing software. You will find a lot of free 1D FFT code in the
internet. I sometimes use a macro that comes with Kaleidagraph that
in turn can be use in a demo mode for free (Mac & Windows versions
available).

I suggest to export the line data etc.

If precision and computational effort is not an issue you may even
apply a 2D FFT to the [time-] line (set the rest of the image [slice]
to zero) and you will get the Fourier spectrum of this line projected
in one dimension.


HTH

Herbie

[Stephan Preibisch]

Hi Tom,

I have a FFT Plugin for ImageJ on my homepage:

http://fly.mpi-cbg.de/~preibisch/software.html

It works on 2D and 3D data generating Log-Power and Phase Spectrum, if
this is not exactly what you need you should be able to modify it
accordingly.

All the best!
Stephan

-----Original Message-----
Date:    Mon, 21 Apr 2008 11:55:56 -0500
From:    "Moninger, Thomas" <[hidden email]>
Subject: FFT on  tme series


Group,

I have a time series of 128x128 pixel images that I would like to do a
pixel by pixel FFT,  ideally generating a bell curve/power spectrum
showing the frequency of intensity fluctuation. They are transmitted
light images of beating cilia.

Can't seem to figure out how to do this....any ideas?

Thank you,

Tom

Thomas Moninger [hidden email]
University of Iowa Central Microscopy Research Facilities
www.uiowa.edu/~cemrf

[TDV]

Hi,

How do I overlay a red and a blue image to obtain a third image with
colocalized pixels in imageJ version 1.45s?  It seems there is no longer the RGB Merge function...

Thanks!