PSF

[john ferguson (BI)]

Hello,

I’m wanting to do deconvolution using a measured PSF obtained under the same conditions I will use to do my imaging. To build the point spread function I have taken 30 images of subresolution beads but I am unsure of the best way to combine these images. Are they simply aligned on the x,y and z and then averaged or is there other image processing that occurs at this stage? Has anything been written for imagej?

Thanks for your help.

John