Cells to Individual Images Plugin?

[gabejackson]

Hello!

I was wondering if we have some sort of plugin to create individual images out of cells on a b/w thresholded image? Basically I just got an image from fluorescence microscopy and have the cells are seperated and would now like to create an image out of each cell instead of having them all on one image. Which algorithm or plugin?

Many thanks in advance for any help,

Greetings,

Gabe

[BenTupper]

Hi,

The macro below will create a subimage for each particle listed in the  
results table.  If you don't have a recent copy of ImageJ you'll have  
to modify this line...

run("Specify...", "width=&bw height=&bh x=&bx y=&by");

to look something like this...

run("Specify...", "width="+bw+"height="+bh+"x="+bx+"y="+by");

Cheers,
Ben

run("Blobs (25K)");
origID = getImageID();
setAutoThreshold();
run("Set Measurements...", "  bounding display redirect=blobs.gif  
decimal=3");
run("Analyze Particles...", "size=0-Infinity circularity=0.00-1.00  
show=Nothing clear display record");

for (i=0; i<nResults(); i++){
        selectImage(origID);
        bx = getResult("BX",i);
        by = getResult("BY",i);
        bw = getResult("Width",i);
        bh = getResult("Height",i);
        run("Specify...", "width=&bw height=&bh x=&bx y=&by");
        run("Copy");
        run("Internal Clipboard");
        rename("sub-"+i);
}

On Jan 19, 2010, at 7:54 AM, gabejackson wrote:

> Hello!
>
> I was wondering if we have some sort of plugin to create individual  
> images
> out of cells on a b/w thresholded image? Basically I just got an  
> image from
> fluorescence microscopy and have the cells are seperated and would  
> now like
> to create an image out of each cell instead of having them all on  
> one image.
> Which algorithm or plugin?
>
> Many thanks in advance for any help,
>
> Greetings,
>
> Gabe
> --
> View this message in context: http://n2.nabble.com/Cells-to-Individual-Images-Plugin-tp4419810p4419810.html
> Sent from the ImageJ mailing list archive at Nabble.com.

... [show rest of quote]

Cheers,
Ben

[Fabrice Senger]

gabejackson a écrit :

> Hello!
>
> I was wondering if we have some sort of plugin to create individual images
> out of cells on a b/w thresholded image? Basically I just got an image from
> fluorescence microscopy and have the cells are seperated and would now like
> to create an image out of each cell instead of having them all on one image.
> Which algorithm or plugin?
>
> Many thanks in advance for any help,
>
> Greetings,
>
> Gabe
>  

Hi there,
if you use analyse particles on your segmented image, with the option
add to ROI manager, each cell will appear in the ROI manager, if you
next run taht macro :

run("Misc...", "divide=Infinity hide");





title = getTitle;



// Get path to result directory

  result = getDirectory("result");





// Create a directory in temp

  myDir = result+title+File.separator;

  File.makeDirectory(myDir);

 



 setBatchMode(true);



myID = getImageID();

 n = roiManager("count");

 for (i=0; i<n; i++) {

     selectImage(myID);

     roiManager("select", i);

     run("Duplicate...", "duplicate range=0"  );

saveAs("tiff", myDir+getTitle+"_"+i+1);

run("Clear Outside");

 }

You will create a folder of single cells...

Fabrice.

[gabejackson]

Awesome, thanks a million guys. works like a charm!

Greetings from Switzerland,

Gabe