The radial intensity distribution I(r) of the flourescence dot was fit for each frame with a nonlinear Levenberg-Marquardt routine to the Gaussian:I(r)=Io*exp(-R^2/w^2) +BG, where R is the distance of each pixel to the center of mass. The fitting parameters are Io, w, and BG, where Io is the peak intensity, BG is the background intensity, and w is the measure of the width (the Gauss width). It is very important for us to get the peak intensity and width. some people suggest me to use Plot Profile(Analyze -> Plot.
But I think the "plot profile" is not good. Because the concept of "plot profile"---Displays a two-dimensional graph of the intensities of pixels along a line within the image. The x-axis represents distance along the line and the y-axis is the pixel intensity. For rectangular selections, displays a "column average plot", where the x-axis represents the horizontal distance through the selection and the y-axis the vertically averaged pixel intensity
> ----------
> From: Karan Kampani
> Reply To: List IMAGEJ
> Sent: Monday, April 24, 2006 12:38 PM
> To: List IMAGEJ
> Subject: Particle Intensity Distribution
>
> Hi,
>
> I am new to the group and was wondering if anyone could help me with something I was trying. I need to find the distribution of light intensity from spots in the image . Tried the analyse partcles option and got the count of bright spots in the image but all the means were 0.
> What should I do?
>
> Karan
>
>
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