Dear list members:
I am to be involved into a project working on counting living and dead bacteria (colored red and green by some substance) on images obtained with a microscope. I have a lot of experience on using ImageJ on x-rays, single images, CT stacks and so on, but have never worked with particle counting on color images. I know there are some plugins but I am not sure which and how I should use them.
The images have black background and red and green ovals (bacterias), but there are some problems on counting them automatically:
- the red color contains a lot of green
- bacteria outside the sharp layer are visible (but smeared out)
- some fields with red og green shadow around bacteria groups
I have some ideas how to solve the problems. But I wonder if anybody who has experience with such work could give me advice about literature. Textbooks or papers which could give me some background of the field and methods.
Thank you
Mvh
Gerald R. Torgersen
IT-seksjonen,
Det odontologiske fakultet / Faculty of Dentistry
Universitetet i Oslo / University of Oslo
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