Dear ImageJ users,
I am trying to apply a particle analysis on an osteoclast image. I
encountered some problems with the results which are produced by this
analysis. The problem lie in the min and max values calculated: these
values are equal to zero...
ImageJ also write the following log message:
The display range must first be updated
using Image>Adjust>Brightness/Contrast
or threshold levels defined using
Image>Adjust>Threshold.
Here is the code I am using :
> // open image
> this.imp = IJ.openImage(file.getAbsolutePath());
>
> // image processes
> ContrastEnhancer contrastEnhancer = new ContrastEnhancer();
> contrastEnhancer.stretchHistogram(imp, 0.35);
>
> LutApplier lutApplier = new LutApplier();
> lutApplier.setup("", imp);
> lutApplier.run(imp.getProcessor());
>
> imp.getProcessor().setAutoThreshold(Method.Default, false, ImageProcessor.BLACK_AND_WHITE_LUT);
> imp.getProcessor().autoThreshold();
>
> ResultsTable paResults = new ResultsTable();
> ParticleAnalyzer particleAnalyzer = new ParticleAnalyzer(
> ParticleAnalyzer.IN_SITU_SHOW | ParticleAnalyzer.SHOW_OUTLINES,
> Measurements.AREA | Measurements.MEAN | Measurements.MIN_MAX,
> paResults,
> 0.0,
> INFINITY
> );
> particleAnalyzer.analyze(imp);
> paResults.saveAs(resFilePath);
Can you see any problem ?
Best Regards,
David
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