Dear David,
We had the same problem a while ago with our fluorescent images.
The cells in two channels were shifted a few pixels in x and y direction
due to the optical alignment of the system.
We used the color functions plugin: align RGB planes.
By using this tool it is rather easy to shift manually one channel pixel
by pixel with respect to the other.
Though if you have a lot of images this is not an ideal solution.
Gabriel Landini made this plugin, for download:
http://imagejdocu.tudor.lu/imagej-documenation-wiki/plugins/align_rgb_planes
Personally I would like to know if there is also some kind of plugin or
algorithm to do it automatically.
Good luck,
Christian Breukers
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Biophysical Engineering Group
Building 28 Horst
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-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
David Knecht-charter
Sent: maandag 2 oktober 2006 3:45
To:
[hidden email]
Subject: Pixel shift/image registration
I have what appears to be a pixel shift between red and green
fluorescence images. I am trying to track down the source, but in
the meantime, is there an easy way in ImageJ to manually shift one
image a few pixels relative to the other to register them before/
after merging? It would be nice to be able to do this on the RGB
merged image, but I can't find any way to move one plane relative the
other as you can in Photoshop. Thanks- Dave
Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)
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