Plugin for viewing multiple TIFF files all on one screen? (Cell time-lapse staining)

[acperry]

Hey, so I have a sets of 20-30 images I want to view all at once on one screen for qualitative comparison (cell time-lapse staining) and was wondering if there was any plugin to make that easier aside opening the tiff images -> "tile-ing" --> then dragging the images around on my screen?

I have a DIC and a fluorescent image for each time interval and it is a real pain having to stagger the rows as such to make a proper comparison every time I want to compare sets. Ideally I would like to have them organized as such that I can take an image of the sets (screenshot/ neat figure) to show my PI of my results.

If anyone knows of such a plugin it would be greatly appreciated.

[John Hayes]

Hi,

If you have a stack of these images you can make a montage image with the Stack->Montage command which will look similar to the tiling you describe. Also check out the Stack->Label command to potentially put timestamps if you like. Finally, I believe Stacks->Tools->Combine will allow you to combine a DIC and fluorescent image at each time from a DIC stack and fluorescent stack before labeling and making a montage.

HTH,

John

Le 23 févr. 2015 à 16:53, acperry a écrit :

> Hey, so I have a sets of 20-30 images I want to view all at once on one
> screen for qualitative comparison (cell time-lapse staining) and was
> wondering if there was any plugin to make that easier aside opening tiff the
> tiff images -> "tile-ing" --> then dragging the images around on my screen?
>
> I have a DIC and a fluorescent image for each time interval and it is a real
> pain having to stagger the rows as such to make a proper comparison every
> time I want to compare sets. Ideally I would like to have them organized as
> such that I can take an image of the sets (screenshot/ neat figure) to show
> my PI of my results.
>
> If anyone knows of such a plugin it would be greatly appreciated.
>
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Plugin-for-viewing-multiple-TIFF-files-all-on-one-screen-Cell-time-lapse-staining-tp5011740.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[acperry]

Hey John,

Unfortunately they are not stacks and were taken manually. This is due to the nature of the the isolated cells and the time interval (2h). They tend to "flatten" overtime resulting in the need to re-focus manually.

I suppose I could re-organize them into stacks?

[John Hayes]

You can make them into stacks at least a couple ways. One way is to try File->Import->Image Sequence. Another way is to open them in separate image windows and use Image->Stacks->Images to Stack.  

Once you have the DIC images in a stack and order you like, I suggest you save it and do the same for a fluorescent stack. Then, record a macro that takes the two image stacks as input, labels them and arranges them in the montage/'neat' view that you like so that you can reuse the macro for other datasets.

HTH,

John

Le 23 févr. 2015 à 18:06, acperry a écrit :

> Hey John,
>
> Unfortunately they are not stacks and were taken manually. This is due to
> the nature of the the isolated cells and the time interval (2h). They tend
> to "flatten" overtime resulting in the need to re-focus manually.
>
> I suppose I could re-organize them into stacks?
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Plugin-for-viewing-multiple-TIFF-files-all-on-one-screen-Cell-time-lapse-staining-tp5011740p5011744.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[acperry]

Alright awesome. Thanks so much!

[acperry]

Hey John,

I was finishing up with my QE2 application and was wondering if you put headings and what referencing style you used for the application? Using adobe reader, it seems that I can't bold, italicize, or superscript anything so my proposal is one big block of text. Should I bother breaking it up into headings still? I have some space left.

Thanks,

Alex

On Mon, Feb 23, 2015 at 8:53 PM, acperry [via ImageJ] <[hidden email]> wrote:

Alright awesome. Thanks so much!

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