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Hello,
I have a stack of 400 images of 16 bits unsigned (channel 1) and a stack of 400 images of 16 bits unsigned (channel 2). I want to merge the two channels together color coded red and green respectively.
From what I've been doing, the built in color -> merge function:
1. converts the images to 8 bits
2. It normalizes each image prior to merging
My primary concern is #2.
From what I see, it seems that each image is normalized before merging. This means that an intensity of 255 from image 1 of channel 1, does not correspond to an intensity of 255 from image 2 of channel 1. The problem with the current way of the color -> merge is that an image of no signal, once merged, will have the noise signals amplified to 255 (for instance for 8 bits).
Please let me know how I can merge images without individual normalization, but instead a stack normalization.
P.S. I know there is a stack normalization plugin, but after i use that, I still need to use color -> merge to merge the two stacks together... =(
Any help will be greatly appreciated. Thanks
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