Problem with automated file saving

[Robert Niestroj-Pahl]

Hi there,

I am using ImageJ for a while but only now diving deeper in IJM macro
language.
My Problem: I want to save the "Summary" file output from my macro to a text
file.
The macro works but no text file is generated. I tried several variations,
but nothing worked. Here is my macro:

run("Close All");
path = File.openDialog("Choose a File");
open(path)
macro "DAPI cell count - 20X" {
        run("Unsharp Mask...", "radius=1 mask=0.60");
        run("Subtract Background...", "rolling=50");
        run("8-bit");
        setAutoThreshold("Triangle dark");
        //run("Threshold...");
        //setThreshold(50, 255);
        setOption("BlackBackground", false);
        run("Convert to Mask");
        run("Watershed");
        run("Analyze Particles...", "size=100-Infinity display clear summarize
add");
        close();
path = File.openDialog("Choose a File");
open(path)
macro "Count particles in ROI - 20X" {
original = getImageID()
run("Duplicate...", " ");
copy = getImageID();
selectImage(copy);
run("Median...", "radius=2");
selectImage(original)
imageCalculator("Subtract", original,copy);
run("Auto Threshold", "method=Triangle dark");
run("From ROI Manager");
name1 = getTitle;
            dotIndex = indexOf(name1, ".");
            title1 = substring(name1, 0, dotIndex);
n = roiManager("count");
        for (i=0; i<n; i++)  {
        roiManager("select", i);
         run("Analyze Particles...", "size=1-30 circularity=0.00-1.00
show=Nothing display summarize");}
selectWindow("Summary");
saveAs("Text",title1);
run("Close All");

Thanks for help!

Robert



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[Michael Schmid]

Hi Robert,

you did not specify a path in the SaveAs command, so the file will go to
some default directory.

You will probably find it in the ImageJ directory (if it you have write
access there; otherwise the file might get lost) or maybe your home
directory.


Michael
________________________________________________________________
On 11/04/2018 14:42, Robert Niestroj-Pahl wrote:

> Hi there,
>
> I am using ImageJ for a while but only now diving deeper in IJM macro
> language.
> My Problem: I want to save the "Summary" file output from my macro to a text
> file.
> The macro works but no text file is generated. I tried several variations,
> but nothing worked. Here is my macro:
>
> run("Close All");
> path = File.openDialog("Choose a File");
> open(path)
> macro "DAPI cell count - 20X" {
> run("Unsharp Mask...", "radius=1 mask=0.60");
> run("Subtract Background...", "rolling=50");
> run("8-bit");
> setAutoThreshold("Triangle dark");
> //run("Threshold...");
> //setThreshold(50, 255);
> setOption("BlackBackground", false);
> run("Convert to Mask");
> run("Watershed");
> run("Analyze Particles...", "size=100-Infinity display clear summarize
> add");
> close();
> path = File.openDialog("Choose a File");
> open(path)
> macro "Count particles in ROI - 20X" {
> original = getImageID()
> run("Duplicate...", " ");
> copy = getImageID();
> selectImage(copy);
> run("Median...", "radius=2");
> selectImage(original)
> imageCalculator("Subtract", original,copy);
> run("Auto Threshold", "method=Triangle dark");
> run("From ROI Manager");
> name1 = getTitle;
>              dotIndex = indexOf(name1, ".");
>              title1 = substring(name1, 0, dotIndex);
> n = roiManager("count");
>          for (i=0; i<n; i++)  {
>          roiManager("select", i);
>           run("Analyze Particles...", "size=1-30 circularity=0.00-1.00
> show=Nothing display summarize");}
> selectWindow("Summary");
> saveAs("Text",title1);
> run("Close All");
>
> Thanks for help!
>
> Robert
>
>
>
> --
> Sent from: http://imagej.1557.x6.nabble.com/
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[Swayne, Theresa C.]

Hi Robert,

I think the problem is that the Summary window is actually called “Summary of [image name].”
Here’s a version of your macro that works for me.

***Make sure to copy the comments at the top and keep them at the very top of the macro. ***
These “script parameters” are a handy way to collect file and folder paths.
More info: https://imagej.net/Script_Parameters

// @File(label = "Input image 1", style = "file") path1
// @File(label = "Input image 2", style = "file") path2
// @File(label = "Output directory", style = "directory") outputDir

run("Close All");
//path = File.openDialog("Choose a File");
open(path1)
macro "DAPI cell count - 20X" {
            run("Unsharp Mask...", "radius=1 mask=0.60");
            run("Subtract Background...", "rolling=50");
            run("8-bit");
            setAutoThreshold("Triangle dark");
            //run("Threshold...");
            //setThreshold(50, 255);
            setOption("BlackBackground", false);
            run("Convert to Mask");
            run("Watershed");
            run("Analyze Particles...", "size=100-Infinity display clear summarize add");
            close();
//path = File.openDialog("Choose a File");
open(path2)
macro "Count particles in ROI - 20X" {
original = getImageID()
run("Duplicate...", " ");
copy = getImageID();
selectImage(copy);
run("Median...", "radius=2");
selectImage(original)
imageCalculator("Subtract", original,copy);
run("Auto Threshold", "method=Triangle dark");
run("From ROI Manager");
name1 = getTitle;
            dotIndex = indexOf(name1, ".");
            title1 = substring(name1, 0, dotIndex);
n = roiManager("count");
        for (i=0; i<n; i++)  {
        roiManager("select", i);
         run("Analyze Particles...", "size=1-30 circularity=0.00-1.00 show=Nothing display summarize");}
selectWindow("Summary of "+name1);
saveAs("Text", outputDir + File.separator + title1 + ".txt");
run("Close All");

Hope this helps.
Theresa

------------------------------------
Theresa Swayne, Ph.D.
Associate Research Scientist
Manager, Confocal and Specialized Microscopy Shared Resource<http://hiccc.columbia.edu/research/sharedresources/confocal>

Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
1130 St. Nicholas Ave., Room 222A
New York, NY 10032
Phone: [phone]
[hidden email]<mailto:[hidden email]>


From: ImageJ Interest Group <[hidden email]> on behalf of Robert Niestroj-Pahl <[hidden email]>
Reply-To: "[hidden email]" <[hidden email]>
Date: Wednesday, April 11, 2018 at 8:54 AM
To: "[hidden email]" <[hidden email]>
Subject: Problem with automated file saving

Hi there,

I am using ImageJ for a while but only now diving deeper in IJM macro
language.
My Problem: I want to save the "Summary" file output from my macro to a text
file.
The macro works but no text file is generated. I tried several variations,
but nothing worked. Here is my macro:

run("Close All");
path = File.openDialog("Choose a File");
open(path)
macro "DAPI cell count - 20X" {
                run("Unsharp Mask...", "radius=1 mask=0.60");
                run("Subtract Background...", "rolling=50");
                run("8-bit");
                setAutoThreshold("Triangle dark");
                //run("Threshold...");
                //setThreshold(50, 255);
                setOption("BlackBackground", false);
                run("Convert to Mask");
                run("Watershed");
                run("Analyze Particles...", "size=100-Infinity display clear summarize
add");
                close();
path = File.openDialog("Choose a File");
open(path)
macro "Count particles in ROI - 20X" {
original = getImageID()
run("Duplicate...", " ");
copy = getImageID();
selectImage(copy);
run("Median...", "radius=2");
selectImage(original)
imageCalculator("Subtract", original,copy);
run("Auto Threshold", "method=Triangle dark");
run("From ROI Manager");
name1 = getTitle;
            dotIndex = indexOf(name1, ".");
            title1 = substring(name1, 0, dotIndex);
n = roiManager("count");
        for (i=0; i<n; i++)  {
        roiManager("select", i);
         run("Analyze Particles...", "size=1-30 circularity=0.00-1.00
show=Nothing display summarize");}
selectWindow("Summary");
saveAs("Text",title1);
run("Close All");

Thanks for help!

Robert



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[Wayne Rasband-2]

> On Apr 11, 2018, at 8:42 AM, Robert Niestroj-Pahl <[hidden email]> wrote:
>
> Hi there,
>
> I am using ImageJ for a while but only now diving deeper in IJM macro
> language.
> My Problem: I want to save the "Summary" file output from my macro to a text
> file.
> The macro works but no text file is generated. I tried several variations,
> but nothing worked. Here is my macro:

The saveAs() function is not passed a full file path so it’s probably saving the text in an unexpected directory. The following example saves the Summary table as a text file using a full file path.

-wayne

  open("");
  dir = File.directory;
  name = File.nameWithoutExtension;
  savePath = dir + name + ".txt";
  setAutoThreshold("Default");
  run("Analyze Particles...", "size=1-30 display summarize");
  saveAs("Text", savePath);
  print("name = "+savePath);
  print("dir = "+dir);
  print("save path = "+savePath);
  open(savePath);
 

>
> run("Close All");
> path = File.openDialog("Choose a File");
> open(path)
> macro "DAPI cell count - 20X" {
> run("Unsharp Mask...", "radius=1 mask=0.60");
> run("Subtract Background...", "rolling=50");
> run("8-bit");
> setAutoThreshold("Triangle dark");
> //run("Threshold...");
> //setThreshold(50, 255);
> setOption("BlackBackground", false);
> run("Convert to Mask");
> run("Watershed");
> run("Analyze Particles...", "size=100-Infinity display clear summarize
> add");
> close();
> path = File.openDialog("Choose a File");
> open(path)
> macro "Count particles in ROI - 20X" {
> original = getImageID()
> run("Duplicate...", " ");
> copy = getImageID();
> selectImage(copy);
> run("Median...", "radius=2");
> selectImage(original)
> imageCalculator("Subtract", original,copy);
> run("Auto Threshold", "method=Triangle dark");
> run("From ROI Manager");
> name1 = getTitle;
>            dotIndex = indexOf(name1, ".");
>            title1 = substring(name1, 0, dotIndex);
> n = roiManager("count");
>        for (i=0; i<n; i++)  {
>        roiManager("select", i);
>         run("Analyze Particles...", "size=1-30 circularity=0.00-1.00
> show=Nothing display summarize");}
> selectWindow("Summary");
> saveAs("Text",title1);
> run("Close All");
>
> Thanks for help!
>
> Robert
>
>
> --
> Sent from: http://imagej.1557.x6.nabble.com/

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[Robert Niestroj-Pahl]

Hi Theresa,
many thanks, that is indeed a very handy way to handle paths!
But still, I have a problem.
I tried the macro that you wrote, but ImageJ (actually it's Fiji) said:
/There is no window with the name Summary of.../
Then I changed the line to selectWindow("Summary"). But then I got another
error message, the one I got before: /ImageJ cannot save when file path both
contains " " and "]"/
I am using MacOS, is there maybe a problem with file access?

Thank you for help, that's a great mailing list! Glad I found this.



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[Robert Niestroj-Pahl]

Hi all,

Problem solved !!!! :-)

I realised that I had indeed some rectangle brackets in my file path...

Now it works!

Robert



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