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Hello,
I am trying to quantify some IF staining in the superficial layers of the spinal cord. I do some some staining and would like compare optical density between two different models.
Am I correct in selecting a region that encompasses the stained layers I-II of the dorsal horn, with the free pen and measuring that, and then dragging that section to an unstained region of the spinal cord and measuring that as the background?
I have used a similar method in the past to quantify molecular weight bands of western blots.
I hope you can help me.
Kind regards,
Mak
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