RGB Profiler values

[Darren David Thomson]

Hi

I'm currently running an experiment that looks at membrane channel  
internalisation over time.

i have a 2 channel image. RED with my membrane protein and GREEN with  
GFP (which stays inside the cell at all times due to being insoluable)

I did an RGB profile of the a line across the middle of the whole of  
cell of a merged G/R image and it gave me a great graph showing the  
RED peaks outside the cell (at time zero) and green peaking inside the  
cell.

I now need to get the number values out of this line that i have drawn  
across the cell. when i click MEASURE it only gives me grey scale  
values, not the separate green and red values.

My aim would be to measure the red values at the membrane compared to  
inside the cell over a time course of incubation.



Thanks for you time,

regards

Darren Thomson
BSc Physiology (hons)
ID:s0971727

--
The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.

[Rasband, Wayne (NIH/NIMH) [E]]

On Jul 6, 2010, at 8:58 AM, Darren David Thomson wrote:

> Hi
>
> I'm currently running an experiment that looks at membrane channel  
> internalisation over time.
>
> i have a 2 channel image. RED with my membrane protein and GREEN with  
> GFP (which stays inside the cell at all times due to being insoluable)
>
> I did an RGB profile of the a line across the middle of the whole of  
> cell of a merged G/R image and it gave me a great graph showing the  
> RED peaks outside the cell (at time zero) and green peaking inside the  
> cell.
>
> I now need to get the number values out of this line that i have drawn  
> across the cell. when i click MEASURE it only gives me grey scale  
> values, not the separate green and red values.
>
> My aim would be to measure the red values at the membrane compared to  
> inside the cell over a time course of incubation.

Convert the RGB image to composite color using Image>Color>Make Composite and the Measure and Plot Profile commands will give separate red and green values. Enable "Display Labels" in Analyze>Set Measurements and, when using the Measure command, the channel ("Red" or "Green") will be shown in the Results table "Label" column.

-wayne