RGB split

[Jessica Widjaja]

 

Hi

My sample is carrot which has orange color and I studied the acid diffusion
into the carrot using blue dye.

On the image that  I took, the outer surrounding layer of the carrot has
green color but the inside is still orange.

 

 How can I quantify how much blue color in my sample since the dye turns
into green on the carrot?

Should I split the image into Red-Green-Blue channel first and measure the
plot profile only from the blue channel?

 

Jessica

[David Webster]

You might want to try Landini's (see plugins page) Color Threshold plugin
applied to HSB(V) images. The Hue channel (H) may give a nice scalar measure
that relates directly to percieved color.

David Webster

On Fri, Sep 4, 2009 at 11:12 AM, Jessica Widjaja <[hidden email]>wrote:

>
>
> Hi
>
> My sample is carrot which has orange color and I studied the acid diffusion
> into the carrot using blue dye.
>
> On the image that  I took, the outer surrounding layer of the carrot has
> green color but the inside is still orange.
>
>
>
>  How can I quantify how much blue color in my sample since the dye turns
> into green on the carrot?
>
> Should I split the image into Red-Green-Blue channel first and measure the
> plot profile only from the blue channel?
>
>
>
> Jessica
>

[Jessica Widjaja]

Hi David,
I can't find the Landini's color threshold under the plugin toolbar.

Jessica

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of David
Webster
Sent: Friday, September 04, 2009 12:43 PM
To: [hidden email]
Subject: Re: RGB split

You might want to try Landini's (see plugins page) Color Threshold plugin
applied to HSB(V) images. The Hue channel (H) may give a nice scalar measure
that relates directly to percieved color.

David Webster

On Fri, Sep 4, 2009 at 11:12 AM, Jessica Widjaja
<[hidden email]>wrote:

>
>
> Hi
>
> My sample is carrot which has orange color and I studied the acid
diffusion

> into the carrot using blue dye.
>
> On the image that  I took, the outer surrounding layer of the carrot has
> green color but the inside is still orange.
>
>
>
>  How can I quantify how much blue color in my sample since the dye turns
> into green on the carrot?
>
> Should I split the image into Red-Green-Blue channel first and measure the
> plot profile only from the blue channel?
>
>
>
> Jessica
>

[Jan Eglinger-5]

Hi Jessica,

Jessica Widjaja wrote:
> I can't find the Landini's color threshold under the plugin toolbar.


I suppose David meant the "Threshold_Colour" plugin in Gabriel Landini's
plugin collection

http://www.dentistry.bham.ac.uk/landinig/software/software.html

that is linked from the main ImageJ plugin page

http://rsbweb.nih.gov/ij/plugins/index.html


Jan


>
> You might want to try Landini's (see plugins page) Color Threshold plugin
> applied to HSB(V) images. The Hue channel (H) may give a nice scalar measure
> that relates directly to percieved color.
>

[David Webster]

Whoops! That's  what I meant. Anyway, when you run it, it displays a window
with HSb(orV), RGB, or etc histograms with sliders for thresholding. You can
threshold, or slice on these histograms and see the result in your image.

On Fri, Sep 4, 2009 at 1:38 PM, Jan Eglinger <[hidden email]> wrote:

> Hi Jessica,
>
> Jessica Widjaja wrote:
> > I can't find the Landini's color threshold under the plugin toolbar.
>
>
> I suppose David meant the "Threshold_Colour" plugin in Gabriel Landini's
> plugin collection
>
> http://www.dentistry.bham.ac.uk/landinig/software/software.html
>
> that is linked from the main ImageJ plugin page
>
> http://rsbweb.nih.gov/ij/plugins/index.html
>
>
> Jan
>
>
> >
> > You might want to try Landini's (see plugins page) Color Threshold plugin
> > applied to HSB(V) images. The Hue channel (H) may give a nice scalar
> measure
> > that relates directly to percieved color.
> >
>

[Gabriel Landini]

On Friday 04 September 2009, Jessica Widjaja wrote:
> On the image that  I took, the outer surrounding layer of the carrot has
> green color but the inside is still orange.

Can you post an image in  the web somewhere?
I would be interested to know if that can be solved using colour
deconvolution.
Cheers
G.

[Joel Sheffield]

Jessica,

Do you know if the blue dye is changing its color as the result of a
reaction, or if the green that you see is the result of adding the blue to
the orange?  What, exactly, is the blue dye, and how is it related to acid?
Can you change the blue dye to green without a carrot?

Joel




On Sat, Sep 5, 2009 at 3:28 AM, Gabriel Landini <[hidden email]>wrote:

> On Friday 04 September 2009, Jessica Widjaja wrote:
> > On the image that  I took, the outer surrounding layer of the carrot has
> > green color but the inside is still orange.
>
> Can you post an image in  the web somewhere?
> I would be interested to know if that can be solved using colour
> deconvolution.
> Cheers
> G.
>

--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs <http://astro.temple.edu/%7Ejbs>

[Jessica Widjaja]

Hi Joel,
The blue dye that I used is methylene blue powder. The blue color only
changes to green when it is diffused into carrot.
When I did the experiment with whey protein gel which has white color, the
dye is still blue.
The blue dye does not react with acid since when I mix it with the acid, the
color of the acid becomes dark blue.

Jessica

On Sat, Sep 5, 2009 at 6:06 PM, JOEL B. SHEFFIELD <[hidden email]> wrote:

> Jessica,
>
> Do you know if the blue dye is changing its color as the result of a
> reaction, or if the green that you see is the result of adding the blue to
> the orange?  What, exactly, is the blue dye, and how is it related to acid?
> Can you change the blue dye to green without a carrot?
>
> Joel
>
>
>
>
> On Sat, Sep 5, 2009 at 3:28 AM, Gabriel Landini <[hidden email]
> >wrote:
>
> > On Friday 04 September 2009, Jessica Widjaja wrote:
> > > On the image that  I took, the outer surrounding layer of the carrot
> has
> > > green color but the inside is still orange.
> >
> > Can you post an image in  the web somewhere?
> > I would be interested to know if that can be solved using colour
> > deconvolution.
> > Cheers
> > G.
> >
>
>
>
> --
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [hidden email]
> URL:  http://astro.temple.edu/~jbs <http://astro.temple.edu/%7Ejbs> <
> http://astro.temple.edu/%7Ejbs>
>

--

Jessica M. Widjaja
Graduate Student Research Assistant,
Biological Systems Engineering
Food Engineering specialization
University of California, Davis 2010
Email: [hidden email]

[Jessica Widjaja]

Hello Gabriel,
I can not post the image that I took in the web.
Is it possible to send to your email directly?

Thank you,
Jessica

On Sat, Sep 5, 2009 at 12:28 AM, Gabriel Landini <[hidden email]>wrote:

> On Friday 04 September 2009, Jessica Widjaja wrote:
> > On the image that  I took, the outer surrounding layer of the carrot has
> > green color but the inside is still orange.
>
> Can you post an image in  the web somewhere?
> I would be interested to know if that can be solved using colour
> deconvolution.
> Cheers
> G.
>

--

Jessica M. Widjaja
Graduate Student Research Assistant,
Biological Systems Engineering
Food Engineering specialization
University of California, Davis 2010
Email: [hidden email]