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Dear ImageJ users.
I am currently using Fiji, which works the same as image J, except it is provided with a bunch of plugins.
I try to estimate the average fluorescence of samples which have been marked using fluorescent antibodies.
So far, I am quite happy with the results...
... but anyway, while trying to improve my model, I found a plugin in : Image > Color > RGB to Luminance ; and which also gives pretty good results.
I guess the plugin transforms my RGB image to gray-scale using the formula : Y = 0,299 R + 0,587 G + 0,114 B.
However, I'd like to be sure about that.
Could anyone confirm ?
There is actually no documentation available on Fiji's website ; and I didn't manage to find the jar file on my computer...
Thanks in advance for your answers.
Valerian
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