Radiographic density

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Radiographic density

deluxe2471
Hello ImageJ experts!

I have just started using ImageJ for the first time. I want to use ImageJ
for bone density measurements in radiographs with a reference aluminum step wedge. I need to calibrate the images to the step wedge to adjust for radiographic differences in exposure.  I then need to measure the density of specific ROI.  I have been reading manuals to accomplish this, but I don't think I going down the right road.  Any help would be greatly appreicated as this is part of my masters project.  

Thank you very much in advance.