Re: Cell counter plugin..

[Oran Kennedy]

Dear List,

 

I am using the 'Cell Counter' plugin at present to analyse histological bone
sections - Rather than choosing the colour and then clicking POINTS on the
image I would like to be able to choose a colour and then trace out a
PERIMETER with that colour (with a standard pixel width). Is there a
different plugin that does this or can the present one be adjusted??

 

Also, how would I go about adding another colour option?

 

Any comments would be greatly appreciated,

 

Thanks,

 

Oran  

 

Oran Kennedy

Department of Anatomy

Royal College Surgeons in Ireland

123 St. Stephen's Green

Dublin 2

*    01 402 2147
*    01 402 2355
*   [hidden email]
*    http://www.rcsi.ie <http://www.rcsi.ie/>    

 

[Andrew Kligerman]

I don't know if this will help, but I am doing cell counting on
hepatocyte and kidney sections with my own macro, and what I found
helpful is to split the color images into their respective red, green,
and blue slices and choose the color that has the most contrast for the
cells I want, close up the other slices , and count the one with the
best contrast.

Andy Kligerman
US EPA

[Oran Kennedy]

Hello again,

 

Thanks for that suggestion, it makes things slightly better - However, I'm
still interested in a plug-in that allows me to trace out a line with each
of the colours....any ideas would be greatly appreciated!

 

Thanks,

 

Oran

 

  _____  

From: Oran Kennedy
Sent: 10 November 2005 10:29
To: '[hidden email]'
Subject: RE: Cell counter plugin..

 

 

I don't know if this will help, but I am doing cell counting on hepatocyte
and kidney sections with my own macro, and what I found helpful is to split
the color images into their respective red, green, and blue slices and
choose the color that has the most contrast for the cells I want, close up
the other slices , and count the one with the best contrast.

 

Andy Kligerman

US EPA

 

 

  _____  

From: Oran Kennedy
Sent: 10 November 2005 10:29
To: '[hidden email]'
Subject: RE: Cell counter plugin..

 

Dear List,

 

I am using the 'Cell Counter' plugin at present to analyse histological bone
sections - Rather than choosing the colour and then clicking POINTS on the
image I would like to be able to choose a colour and then trace out a
PERIMETER with that colour (with a standard pixel width). Is there a
different plugin that does this or can the present one be adjusted??

 

Any comments would be greatly appreciated,

 

Thanks,

 

Oran  

 

Oran Kennedy

Department of Anatomy

Royal College Surgeons in Ireland

123 St. Stephen's Green

Dublin 2

*    01 402 2147
*    01 402 2355
*   [hidden email]
*    http://www.rcsi.ie <http://www.rcsi.ie/>    

 

[Kurt De Vos]

Hi,

It should be possible to do that by changing the plugin to do select  
by wand when you click instead of putting the standard marker, and  
then outline the resulting ROI.
I can have a look but it might take a while to get around to.
kurt

On 11 Nov 2005, at 19:10, Oran Kennedy wrote:

> Hello again,
>
>
>
> Thanks for that suggestion, it makes things slightly better -  
> However, I'm
> still interested in a plug-in that allows me to trace out a line  
> with each
> of the colours....any ideas would be greatly appreciated!
>
>
>
> Thanks,
>
>
>
> Oran
>
>
>
>   _____
>
> From: Oran Kennedy
> Sent: 10 November 2005 10:29
> To: '[hidden email]'
> Subject: RE: Cell counter plugin..
>
>
>
>
>
> I don't know if this will help, but I am doing cell counting on  
> hepatocyte
> and kidney sections with my own macro, and what I found helpful is  
> to split
> the color images into their respective red, green, and blue slices and
> choose the color that has the most contrast for the cells I want,  
> close up
> the other slices , and count the one with the best contrast.
>
>
>
> Andy Kligerman
>
> US EPA
>
>
>
>
>
>   _____
>
> From: Oran Kennedy
> Sent: 10 November 2005 10:29
> To: '[hidden email]'
> Subject: RE: Cell counter plugin..
>
>
>
> Dear List,
>
>
>
> I am using the 'Cell Counter' plugin at present to analyse  
> histological bone
> sections - Rather than choosing the colour and then clicking POINTS  
> on the
> image I would like to be able to choose a colour and then trace out a
> PERIMETER with that colour (with a standard pixel width). Is there a
> different plugin that does this or can the present one be adjusted??
>
>
>
> Any comments would be greatly appreciated,
>
>
>
> Thanks,
>
>
>
> Oran
>
>
>
> Oran Kennedy
>
> Department of Anatomy
>
> Royal College Surgeons in Ireland
>
> 123 St. Stephen's Green
>
> Dublin 2
>
> *    01 402 2147
> *    01 402 2355
> *   [hidden email]
> *    http://www.rcsi.ie <http://www.rcsi.ie/>
>
>

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