Re: [Janelia Farm] Can ImageJ calculate fiber orientation

[Albert Cardona-2]

> Hi Dr. Cardona,
>
> After searching, there are so many websites about your ImageJ tutorials. I'm
> impressed.
>
> My Ph.D. research is about stem cell human mesenchymal cells (hMSC). I've
> been using DAPI (blue for nucleus) and Rhodamine-Phalloidin (red for F-actin)
> and I use ImageJ to combine images together to see both nucleus and fibers in
> one image.
>
> To understand in more details, I want to (1) calculate (or count) the number
> of the nucleus and (2) calculate fiber orientation or how well these
> microfibers align in one direction compared to others (or in each direction).
>
> So, I wondered if I can use ImageJ to do that. If ImageJ can do so, how can I
> do that?
>
> Thank you for your help.
> Suphannee
>
>

Suphannee,

I suggest starting with the ImageJ documentation:

http://rsbweb.nih.gov/ij/docs/index.html

... and in particular with the user guide:

http://rsbweb.nih.gov/ij/docs/user-guide.pdf


Best,

Albert




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