Re: Skeleton node and branch measurement

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Re: Skeleton node and branch measurement

Sudhakar Jaganathan
Is there a method or plugin to measure number of nodes and branches in the
skeletonized image



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Sudhakar Jaganathan

North Carolina State University
College of Textile
NC-USA
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Re: Skeleton node and branch measurement

Gabriel Landini
On Friday 13 January 2006 04:49, Sudhakar Jaganathan wrote:
> Is there a method or plugin to measure number of nodes and branches in the
> skeletonized image

For the nodes:

BinaryConnectivity_.class
Returns the number of connected pixels (+1) to each foreground pixel (8
neighbours): background = 0, single pixel = 1, end of a line = 2,
bifurcations = 3, triple points = 4, etc. Brightness/Contrast must be
adjusted to see the result.

It is included in the Morphology collection of plugins here:
http://www.dentistry.bham.ac.uk/landinig/software/software.html

For the node branches, I am not sure IJ can measure the length of lines as
done in other imaging programmes (like Optimas, for instance, where one
explicitly must set a 'line analysis mode'). Does anybody know anything about
this?

One could delete the various branching points and measure the number of pixels
in the remaining clusters (this of course would be just an underestimation,
and not very good either [the maximum error factor would be sqrt(2) when all
the pixels are orientated along a diagonal line ]).

Cheers,

Gabriel
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Re: Skeleton node and branch measurement

Andrea
HI, I used skeletonize to asses network between mitochondria.  I=C2=B4m int=
erested in quantify this network - It is this possible and how I have to do=
 it?? Please send me a protocol step by step with ImageJ software. Andrea