Hi Ellen,
> when i drag and drop the files containing the TIFs into image J,
> although it allows me to open the merged image; when i attempt to
> split it am informed that an images 'must be multichannel' in order to
> split.
Try using File > Import > Bio-Formats to open your data. Or if you wish to
drag and drop, run Plugins > LOCI > LOCI Shortcuts Window and drop the
files onto that. Otherwise, dragging and dropping will not use Bio-Formats
to open your data, so you will not receive the option to split channels
during import.
Regards,
Curtis
P.S. I CCed the ImageJ mailing list, since I think you meant to write your
mail there?
On Tue, May 28, 2013 at 4:21 AM, <
[hidden email]> wrote:
> Hello
>
> I am quite new to image j. But until now i have routinely been able to
> select to save confocal images as as TIFs (OIFs instead of the default
> confocal option of OIB). I have then gone to image j which has allowed me
> to select 'split channels' after which i have assigned colours, merged as i
> pleased and so on and so on. But I have confocal stacks which are 4
> channels, and when i drag and drop the files containing the TIFs into image
> J, although it allows me to open the merged image; when i attempt to split
> it am informed that an images 'must be multichannel' in order to split.
>
> Any advice? its taken months to get these images, and the samples are no
> longer usable to re image so i am a little panicked :)
>
> cheers for your help guys!
>
> ellen
>
> _____________________________________
> Sent from
http://imagej.1557.x6.nabble.com>
>
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