opening every nth frame of an AVI into ImageJ

[Cammer, Michael]

We have a few long MP4 files that we want to open into ImageJ.

Using QuicktimePro I converted a few of them to uncompressed AVI.  The frame size is 1280 X 720 RGB and we need to open every 80th frame or so.  Since the files are bigger than 10GB, I figured the easiest way would be to open as raw and make the interval between frames 1280 X 720 X 3 X n where n would be between 60 and 120 inclusive.  However, it doesn't seem so simple as the frames roll and the colors alternate regardless whether we choose RGB or BGR.

Two questions:

1.  Are the frame intervals fixed in an uncompressed AVI and
2. if so, does anyone know the correct header and frame interval values to use?

And a third question:

Is there a better way to do this?

Thank you!

________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]


--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[ctrueden]

Hi Michael,

> we need to open every 80th frame or so

Did you try using the "Specify range for each series" option of Bio-Formats
to set a Step value as desired? Does Bio-Formats read in your AVIs
correctly in general? (I forget whether Bio-Formats currently handles AVIs
>2GB at all. If not, you could try exporting to a different format from
QuickTime.)

Regards,
Curtis



On Tue, May 28, 2013 at 12:47 PM, Cammer, Michael <
[hidden email]> wrote:

> We have a few long MP4 files that we want to open into ImageJ.
>
> Using QuicktimePro I converted a few of them to uncompressed AVI.  The
> frame size is 1280 X 720 RGB and we need to open every 80th frame or so.
>  Since the files are bigger than 10GB, I figured the easiest way would be
> to open as raw and make the interval between frames 1280 X 720 X 3 X n
> where n would be between 60 and 120 inclusive.  However, it doesn't seem so
> simple as the frames roll and the colors alternate regardless whether we
> choose RGB or BGR.
>
> Two questions:
>
> 1.  Are the frame intervals fixed in an uncompressed AVI and
> 2. if so, does anyone know the correct header and frame interval values to
> use?
>
> And a third question:
>
> Is there a better way to do this?
>
> Thank you!
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: [phone]  Cell: [phone]
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Cammer, Michael]

Actually, there's a really easy way to do it that I overlooked.  Quicktime will export to a TIF sequence!
Regards,
Michael


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Curtis Rueden
Sent: Tuesday, May 28, 2013 1:55 PM
To: [hidden email]
Subject: Re: opening every nth frame of an AVI into ImageJ

Hi Michael,

> we need to open every 80th frame or so

Did you try using the "Specify range for each series" option of Bio-Formats to set a Step value as desired? Does Bio-Formats read in your AVIs correctly in general? (I forget whether Bio-Formats currently handles AVIs
>2GB at all. If not, you could try exporting to a different format from
QuickTime.)

Regards,
Curtis



On Tue, May 28, 2013 at 12:47 PM, Cammer, Michael < [hidden email]> wrote:

> We have a few long MP4 files that we want to open into ImageJ.
>
> Using QuicktimePro I converted a few of them to uncompressed AVI.  The
> frame size is 1280 X 720 RGB and we need to open every 80th frame or so.
>  Since the files are bigger than 10GB, I figured the easiest way would
> be to open as raw and make the interval between frames 1280 X 720 X 3
> X n where n would be between 60 and 120 inclusive.  However, it
> doesn't seem so simple as the frames roll and the colors alternate
> regardless whether we choose RGB or BGR.
>
> Two questions:
>
> 1.  Are the frame intervals fixed in an uncompressed AVI and 2. if so,
> does anyone know the correct header and frame interval values to use?
>
> And a third question:
>
> Is there a better way to do this?
>
> Thank you!
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist Skirball Institute of
> Biomolecular Medicine
> Lab: [phone]  Cell: [phone]
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html