Recognize Water Droplets

[Broncosbeatall]

Hi everybody, currently I'm studying condensation of water upon a superhydrophobic surface. I'm relatively new to ImageJ/Fiji and am trying to use it to measure the diameter of water droplets in images such as the one below. My current problem seems to be that the color of the surface and the water droplets are too close to each other, and whenever I try to use threshold to make the image analyzable, the shapes become blurred between themselves and the background, resulting in a deformed bubble. Does anyone have any tips that could help me better differentiate the water droplets from the background?

Thanks in advance

[Rainer M. Engel]

Hi Thomas.. (?),

I'm not a specialist in this area but I would try to optimise in the
real or analogue world before I get frustrated in the digital.

Maybe you are able with other backgrounds and light-setup to get dark
fresnel edges of your droplets.

Just a thought..
Rainer



Am 13.07.2012 21:06, schrieb Broncosbeatall:

> Hi everybody, currently I'm studying condensation of water upon a
> superhydrophobic surface. I'm relatively new to ImageJ/Fiji and am trying to
> use it to measure the diameter of water droplets in images such as the one
> below. My current problem seems to be that the color of the surface and the
> water droplets are too close to each other, and whenever I try to use
> threshold to make the image analyzable, the shapes become blurred between
> themselves and the background, resulting in a deformed bubble. Does anyone
> have any tips that could help me better differentiate the water droplets
> from the background?
>
> Thanks in advance
>
> http://imagej.1557.n6.nabble.com/file/n4999407/Droplet6.jpg 
> http://imagej.1557.n6.nabble.com/file/n4999407/droplet5-1.jpg 
>
> --
> View this message in context: http://imagej.1557.n6.nabble.com/Recognize-Water-Droplets-tp4999407.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
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[Tracy Anderson]

It's all about your lighting.  Have you tried oblique illumination?

Tracy




From:   "Rainer M. Engel" <[hidden email]>
To:     <[hidden email]>
Date:   07/13/2012 02:46 PM
Subject:        Re: Recognize Water Droplets
Sent by:        ImageJ Interest Group <[hidden email]>



Hi Thomas.. (?),

I'm not a specialist in this area but I would try to optimise in the
real or analogue world before I get frustrated in the digital.

Maybe you are able with other backgrounds and light-setup to get dark
fresnel edges of your droplets.

Just a thought..
Rainer



Am 13.07.2012 21:06, schrieb Broncosbeatall:
> Hi everybody, currently I'm studying condensation of water upon a
> superhydrophobic surface. I'm relatively new to ImageJ/Fiji and am
trying to
> use it to measure the diameter of water droplets in images such as the
one
> below. My current problem seems to be that the color of the surface and
the
> water droplets are too close to each other, and whenever I try to use
> threshold to make the image analyzable, the shapes become blurred
between
> themselves and the background, resulting in a deformed bubble. Does
anyone

> have any tips that could help me better differentiate the water droplets
> from the background?
>
> Thanks in advance
>
> http://imagej.1557.n6.nabble.com/file/n4999407/Droplet6.jpg 
> http://imagej.1557.n6.nabble.com/file/n4999407/droplet5-1.jpg 
>
> --
> View this message in context:

http://imagej.1557.n6.nabble.com/Recognize-Water-Droplets-tp4999407.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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