I tried the mask technique. When i create the first mask, it creates a new image. When i create the second, it overlays it on the first! so i cant make an "AND" function since there is only one image?
once i have the new mask (if i can get it to work), do i just measure and take the area of the line as a length or the (perimeter -2)/2?
I also tried the ROI macro method. It nicely produced a single pixel roi of the overlap. Problem is, when i measure it, the perimeter is 175. the area is 42. BUT when i draw a freehand line, it is length 54! i dont get it! which is the real length of the line?
Dear Andy
flettster <drflett@...> writes:
> Im not sure if that will work or not. I have uploaded an image and a roi set.
> I need the length of the line shared by "Endo" and "man".
>
http://dl.dropbox.com/u/1070657/test%20%282%29.zip
Gabriel's suggestion is your best bet.
For that, you would create 2 masks (one for each ROI) using 'Edit>Selection>
Create Mask', run 'Process>Binary>Dilate' on one of the masks, and then
'Process>Image Calculator...' to compute AND between the two masks.
You can, however, perform logical operations on ROIs using the ROI Manager.
The following macro applied to your dataset exemplifies it:
// -------
requires("1.45i");
roiManager("select", 0); // select Endo
run("Enlarge...", "enlarge=1");
setSelectionName("DilatedEndo");
roiManager("add");
roiManager("select", newArray(2,3)); // select man and enlarged Endo
roiManager("AND");
run("Set Measurements...", "perimeter");
run("Measure");
lenght = getResult("Perim.", nResults-1)/2;
showMessage("Abut Region", "Length (perim./2): "+ lenght);
// -------
HTH,
-tiago
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