Rotating Images of DIC stacks?

[Joel Sheffield]

Hi All,

As you know, the stacks operation in ImageJ (and other systems as
well) assumes a stack containing essentially transparent slices with
regions of intensity set either by the fluorescence of samples or by
high contrast imaging.

It has occurred to me that DIC images can also be accumulated as
stacks, and it would be nice to be able to create rotating models of
these stacks as well.

As I understand it (and correct me if I am wrong), the 3D view system
in ImageJ essentially takes a series of maximum projections with the
slices shifted slightly with respect to each other for each frame of
the series.

Max projections of DIC stacks generally look awful.  However, there
are several plugins available (Stack Focusser, Extended Depth of
Field (Focus)) that attempt to create an analog of such a projection.
 Often, these are quite successful.  

So, my question is, has anyone created a macro, or a plugin, that
would take a DIC stack, create a "focussed image", then shift the
elements of the stack and create a new image, etc.?  This could be
used both for anaglyphs and for rotating images.

Joel



--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
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[phone], fax [phone]
http://astro.temple.edu/~jbs