SCALE

[Hughes, Shawna]

Hello,

I am new to the ImageJ software and I have a question, I am working with dough and analyzing air bubbles of the dough with a light microscope. The light microscope does not have a scale and I was wondering how do I get a so that I can start accumulating the results. I will be measuring the area, length, and width.

I really do need help.
Thank you in advance

Shawna Hughes

Shawna Hughes
Graduate Student, Food Science
NSF Bridge to Doctorate Fellow
Advisor: Dr. Rayas-Duarte
Oklahoma State University
Robert M. Kerr Food & Agricultural Products Center
Stillwater, OK 74078

[Colin Rickman-3]

Hi

You need to know two bits of information. The physical size of a pixel on your CCD in your camera and the total magnification between your sample and the detector. Divide the physical pixel size by the magnification and the answer is the size of one of your pixels in the displayed image.

Colin

Dr Colin Rickman
Department of Chemistry (WP 2.03)
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)
Tel: [phone]
Fax: [phone]
http://www.eps.hw.ac.uk/departments/chemistry/cr.htm



On 8 Aug 2010, at 06:42, "Hughes, Shawna" <[hidden email]> wrote:

> Hello,
>
> I am new to the ImageJ software and I have a question, I am working with dough and analyzing air bubbles of the dough with a light microscope. The light microscope does not have a scale and I was wondering how do I get a so that I can start accumulating the results. I will be measuring the area, length, and width.
>
> I really do need help.
> Thank you in advance
>
> Shawna Hughes
>
> Shawna Hughes
> Graduate Student, Food Science
> NSF Bridge to Doctorate Fellow
> Advisor: Dr. Rayas-Duarte
> Oklahoma State University
> Robert M. Kerr Food & Agricultural Products Center
> Stillwater, OK 74078

[Gabriel Landini]

On Sunday 08 August 2010, you wrote:
> I am new to the ImageJ software and I
have a question, I am working with
> dough and analyzing air bubbles of the
dough with a light microscope. The
> light microscope does not have a scale
and I was wondering how do I get a
> so that I can start accumulating the
results. I will be measuring the
> area, length, and width.

This is called
spatial calibration.
You can photograph a stage micrometer a the
magnification you are using and so you know how many pixels fit in a known
distance. From this you know what is the dimension of one pixel (actually,
the inter-pixel dimension because pixels should be considered points).
Then
you enter this value as a spatial calibration in the Image Properties. Then
the results of the measurements can be given in calibrated units. If you
save the images as tiff, the calibration will be saved in them.

This is the
first thing one should do in a new microscope or a new camera (assuming that
it is a fixed camera, without zoom) for all the objectives. You should keep
the values as they can be re-used later on and/or add them to a plugin like
this one, which makes things easier if you deal with several combinations of
microscopes and
cameras.

http://rsb.info.nih.gov/ij/plugins/microscope-scale.html


Cheers

G.

[Hughes, Shawna]

Hi,

Can someone tell me what is a CCD?

Thank you

Shawna Hughes
Graduate Student, Food Science
NSF Bridge to Doctorate Fellow
Advisor: Dr. Rayas-Duarte
Oklahoma State University
Robert M. Kerr Food & Agricultural Products Center
Stillwater, OK 74078
________________________________________
From: ImageJ Interest Group [[hidden email]] On Behalf Of Colin Rickman [[hidden email]]
Sent: Sunday, August 08, 2010 3:50 AM
To: [hidden email]
Subject: Re: SCALE

Hi

You need to know two bits of information. The physical size of a pixel on your CCD in your camera and the total magnification between your sample and the detector. Divide the physical pixel size by the magnification and the answer is the size of one of your pixels in the displayed image.

Colin

Dr Colin Rickman
Department of Chemistry (WP 2.03)
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)
Tel: [phone]
Fax: [phone]
http://www.eps.hw.ac.uk/departments/chemistry/cr.htm



On 8 Aug 2010, at 06:42, "Hughes, Shawna" <[hidden email]> wrote:

> Hello,
>
> I am new to the ImageJ software and I have a question, I am working with dough and analyzing air bubbles of the dough with a light microscope. The light microscope does not have a scale and I was wondering how do I get a so that I can start accumulating the results. I will be measuring the area, length, and width.
>
> I really do need help.
> Thank you in advance
>
> Shawna Hughes
>
> Shawna Hughes
> Graduate Student, Food Science
> NSF Bridge to Doctorate Fellow
> Advisor: Dr. Rayas-Duarte
> Oklahoma State University
> Robert M. Kerr Food & Agricultural Products Center
> Stillwater, OK 74078

[John Oreopoulos]

CCD stands for "charged coupled device". This is your digital camera  
used for capturing images produced by your microscope.

Cheers

John Oreopoulos

On 2010-08-08, at 6:15 PM, "Hughes, Shawna"  
<[hidden email]> wrote:

> Hi,
>
> Can someone tell me what is a CCD?
>
> Thank you
>
> Shawna Hughes
> Graduate Student, Food Science
> NSF Bridge to Doctorate Fellow
> Advisor: Dr. Rayas-Duarte
> Oklahoma State University
> Robert M. Kerr Food & Agricultural Products Center
> Stillwater, OK 74078
> ________________________________________
> From: ImageJ Interest Group [[hidden email]] On Behalf Of Colin  
> Rickman [[hidden email]]
> Sent: Sunday, August 08, 2010 3:50 AM
> To: [hidden email]
> Subject: Re: SCALE
>
> Hi
>
> You need to know two bits of information. The physical size of a  
> pixel on your CCD in your camera and the total magnification between  
> your sample and the detector. Divide the physical pixel size by the  
> magnification and the answer is the size of one of your pixels in  
> the displayed image.
>
> Colin
>
> Dr Colin Rickman
> Department of Chemistry (WP 2.03)
> School of Engineering and Physical Sciences
> Heriot-Watt University
> Edinburgh
> EH14 4AS
>
> Tel: [phone] (Office)
> Tel: [phone]
> Fax: [phone]
> http://www.eps.hw.ac.uk/departments/chemistry/cr.htm
>
>
>
> On 8 Aug 2010, at 06:42, "Hughes, Shawna"  
> <[hidden email]> wrote:
>
>> Hello,
>>
>> I am new to the ImageJ software and I have a question, I am working  
>> with dough and analyzing air bubbles of the dough with a light  
>> microscope. The light microscope does not have a scale and I was  
>> wondering how do I get a so that I can start accumulating the  
>> results. I will be measuring the area, length, and width.
>>
>> I really do need help.
>> Thank you in advance
>>
>> Shawna Hughes
>>
>> Shawna Hughes
>> Graduate Student, Food Science
>> NSF Bridge to Doctorate Fellow
>> Advisor: Dr. Rayas-Duarte
>> Oklahoma State University
>> Robert M. Kerr Food & Agricultural Products Center
>> Stillwater, OK 74078