Hi William,
if you have an 8-bit image and use no compression or PNG compression for
"Save As AVI", the opened image will be an 8-bit monochrome image, and
no compression losses will occur.
If you use JPEG compression, ImageJ will read the avi as RGB image, but
you can select "Convert to Grayscale" when opening the avi file. to get
an 8-bit monochrome image.
Note that saving the avi with JPEG compression will cause compression
artifacts, so it is not meant for analyzing the saved images any
further. JPEG is useful only only for viewing (e.g. embedding in a
presentation).
Michael
________________________________________________________________
On 18.04.21 22:21, William Saidel wrote:
> I have a stack that was originally an RGB video. I used the Image tab>type>8-bit to convert the entire stack, which it did. I saved the new stack as an avi thinking it would be an 8-bit stack when I reloaded. On reloading, the stack was identified as an RGB stack. Is it possible to convert a stack from one type to another and save as the new type?
>
> Prof emeritus Wm Saidel
> Dept of Biology
> Rutgers, the State University of New Jersey
> Camden campus
> Camden, NJ 08102
>
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http://imagej.nih.gov/ij/list.html>
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