Scale image alters pixel information?

[Nicolás Pírez]

Hi all, I have a question.
If I acquire a time lapse with MicroManager and save every frame as a tiff,
and then using ImageJ function Import>Image sequence I am able to have it
as a single tiff.

My question is that if during this process I scale the image (option is in
the Image Sequence Menu window) do I alter in any way the information
(intensity) of the pixels?

Thanks a lot.

Nico Pírez.


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Nicolás Pírez, PhD
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[Jeremy Adler]

It may be faster to test the software yourself - then you really know.

Load your images twice, once with the scale option and once without.
Then compare the results - look at the two final images or see the intensity ranges using analyse/ histogram.

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Nicolás Pírez
Sent: den 30 oktober 2014 19:00
To: [hidden email]
Subject: Scale image alters pixel information?

Hi all, I have a question.
If I acquire a time lapse with MicroManager and save every frame as a tiff, and then using ImageJ function Import>Image sequence I am able to have it as a single tiff.

My question is that if during this process I scale the image (option is in the Image Sequence Menu window) do I alter in any way the information
(intensity) of the pixels?

Thanks a lot.

Nico Pírez.


--
Nicolás Pírez, PhD
[hidden email]
[phone]

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html