Scanner for film autoradiography?

[Ron Hammer]

Can anyone suggest a scanner to use with ImageJ for X-ray film  
autoradiographs? There were earlier references to the Epson 4990  
Photo scanner (4800 X 9600 dpi, 8 X 10" transparency adapter, USB 2.0  
and Firewire); is this still an adequate scanner, or are there  
better? Thanks...

Ron
---
Ronald P. Hammer, Jr., PhD
Professor of Basic Medical Sciences,
   Pharmacology and Psychology
University of Arizona College of Medicine, Phoenix
   in partnership with Arizona State University

Mail: 425 N. Fifth Street
        Phoenix, AZ  85004-2157
Phone: [phone]
FAX:   [phone]
Email: [hidden email]

[Wolfgang Schechinger]

Ron,

we use an quite old HP6100c and HP4c (internal 10bit, external 8bit) with an adaptor for transparencies. Basically any scanner which can handle the film format will do. You may want to use a greywedge (eg available for calibrating densitometers / densities in printing devices or photographic equipment) in any case.

Regards,

Wolfgang



-------- Original-Nachricht --------
> Datum: Mon, 27 Aug 2007 07:03:06 -0700
> Von: Ron Hammer <[hidden email]>
> An: [hidden email]
> Betreff: Scanner for film autoradiography?

> Can anyone suggest a scanner to use with ImageJ for X-ray film  
> autoradiographs? There were earlier references to the Epson 4990  
> Photo scanner (4800 X 9600 dpi, 8 X 10" transparency adapter, USB 2.0  
> and Firewire); is this still an adequate scanner, or are there  
> better? Thanks...
>
> Ron
> ---
> Ronald P. Hammer, Jr., PhD
> Professor of Basic Medical Sciences,
>    Pharmacology and Psychology
> University of Arizona College of Medicine, Phoenix
>    in partnership with Arizona State University
>
> Mail: 425 N. Fifth Street
> Phoenix, AZ  85004-2157
> Phone: [phone]
> FAX:   [phone]
> Email: [hidden email]

--
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[Jim Quinn-2]

Ron and folks

Epson V700 and V750 seem to have replaced the Epson 4990.

I'm happy with my V700.

Jim





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 > Subject: Scanner for film autoradiography?
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 >
 > Can anyone suggest a scanner to use with ImageJ for X-ray film  
 > autoradiographs? There were earlier references to the Epson 4990  
 > Photo scanner (4800 X 9600 dpi, 8 X 10" transparency adapter, USB 2.0  
 > and Firewire); is this still an adequate scanner, or are there  
 > better? Thanks...
 >
 > Ron
 > ---
 > Ronald P. Hammer, Jr., PhD
 > Professor of Basic Medical Sciences,
 >    Pharmacology and Psychology
 > University of Arizona College of Medicine, Phoenix
 >    in partnership with Arizona State University
 >
 > Mail: 425 N. Fifth Street
 > Phoenix, AZ  85004-2157
 > Phone: [phone]
 > FAX:   [phone]
 > Email: [hidden email]
 >

[Daniel Glen]

Depending on what type of autoradiography you're doing, you may be  
interested in spatial resolution, optical density linearity and  
range, and noise. For gels, these are probably less important, but  
for 2-DG (deoxyglucose) metabolism or blood flow autoradiography, you  
might consider a film digitizer. In my former lab several years ago,  
we found a Howtek (now iCAD) film digitizer worked well and gave us  
the capability of feeding it multiple films. We characterized other  
film digitizers also but found poor noise or problems with newton  
ring interference and internal reflections particularly with laser-
based devices.

We also looked at scanners from Agfa and later Epson Expression  
series. Although these are not linear devices and don't have the  
optical density range of the film digitizers, they can have  
equivalent or better spatial resolution and excellent value. Spatial  
resolution can be verified with various optical targets including an  
Stouffer step wedge or a USAF 1951 optical target. Optical density  
step tablets are also available for verifying optical density ranges.  
This kind of thing was originally done with extraordinarily expensive  
and complicated scanning densitometers, but even the current sub-
$1000 devices can probably do a better job.


On Aug 27, 2007, at 10:22 AM, Jim Quinn wrote:

> Ron and folks
>
> Epson V700 and V750 seem to have replaced the Epson 4990.
>
> I'm happy with my V700.
>
> Jim
>
>
>
>
>
>> From [hidden email]  Mon Aug 27 10:16:56 2007
>> Approved-By: [hidden email]
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>> Mime-Version: 1.0 (Apple Message framework v752.3)
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>> X-Virus-Scanned: amavisd-new at email.arizona.edu
>> Content-Transfer-Encoding: 7bit
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>> Date:         Mon, 27 Aug 2007 07:03:06 -0700
>> Reply-To: ImageJ Interest Group <[hidden email]>
>> Sender: ImageJ Interest Group <[hidden email]>
>> From: Ron Hammer <[hidden email]>
>> Subject: Scanner for film autoradiography?
>> To: [hidden email]
>> Precedence: list
>> List-Help: <http://list.nih.gov/cgi-bin/wa?LIST=IMAGEJ>,
>>            <mailto:[hidden email]?body=INFO IMAGEJ>
>> List-Unsubscribe: <mailto:[hidden email]>
>> List-Subscribe: <mailto:[hidden email]>
>> List-Owner: <mailto:[hidden email]>
>> List-Archive: <http://list.nih.gov/cgi-bin/wa?LIST=IMAGEJ>
>>
>> Can anyone suggest a scanner to use with ImageJ for X-ray film
>> autoradiographs? There were earlier references to the Epson 4990
>> Photo scanner (4800 X 9600 dpi, 8 X 10" transparency adapter, USB 2.0
>> and Firewire); is this still an adequate scanner, or are there
>> better? Thanks...
>>
>> Ron
>> ---
>> Ronald P. Hammer, Jr., PhD
>> Professor of Basic Medical Sciences,
>>    Pharmacology and Psychology
>> University of Arizona College of Medicine, Phoenix
>>    in partnership with Arizona State University
>>
>> Mail: 425 N. Fifth Street
>> Phoenix, AZ  85004-2157
>> Phone: [phone]
>> FAX:   [phone]
>> Email: [hidden email]
>>