Hi,
I'm trying to measure neurite outgrowth at different angles. I would like to know if I can config the segmented line tool or straight line tool for this purpose.
Basically, I want to set the length of each segement to be a certain fixed value, say for example, 5 um; and at the same time, the angle of each segement relative to horizontal or vertical axis of the image.
I found this thread regarding set line length, but after I installed the macro it does not seem to function at all, as I can still draw lines exceeding the set length.
http://imagej.588099.n2.nabble.com/how-to-stop-the-freehand-lines-tool-at-a-set-distance-20-um-td4121131.htmlNay help is greatly appriciated.
Chris