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Hi,
I am using Imagej to calculate the thresholded area and intensity of a selected area of staining (endplate receptor cluster and nerve terminal of neuromuscular junction) of two fluorescent channels. There are more than one staining areas (endplates) and I would like to calculate their intensity and area individually. I want to use the same area calculated by thresholding and transfer this area to the other channel.
I can transfer the area surrounding the staining on one channel to the other. I do this by thresholding the channel. Then I use edit -> selection -> create selection.
This creates a 'mask' of the area of the channel that is shown on both channels. However, I cannot use the polygon or freehand selection tools to isolate the area of staining that i would like to consider (individual endplate). When i threshold and measure it calculates the total intensity within all regions of staining (multiple endplates).
Can someone help me with selecting an individual endplate when I have masked certain areas of the image
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