Setting Threshold Value resets display range

[Olivier Burri]

Hi all,

How come when a user is setting the threshold of a 16 bit or 32bit image by hand, this modifies the min and max display range and makes it go back to the default, very often way too dark? This is not the case with 8-bit images...

It makes it rather difficult to visualize whether the threshold was done correctly if a custom display range cannot be set.

Best

Oli

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[Rasband, Wayne (NIH/NIMH) [E]]

On Aug 12, 2015, at 5:00 AM, Burri Olivier <[hidden email]> wrote:
>
> Hi all,
>
> How come when a user is setting the threshold of a 16 bit or 32bit image by hand, this modifies the min and max display range and makes it go back to the default, very often way too dark? This is not the case with 8-bit images...
>
> It makes it rather difficult to visualize whether the threshold was done correctly if a custom display range cannot be set.

The Image>Adjust>Threshold command currently resets the display range of 16 and 32-bit image. As a workaround, make a copy of the the image, convert it to 8-bits, threshold it, set the original image is the “Redirect to” image in the Analyze>Set Measurements dialog, and then using the Analyze Particles command.

-wayne


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