Soma size by Image J

[luciana]

Hi all,
   I want to measure the soma size of neurons immunolabeled. The images of the immunolabeling are saved in tif.
I have images per section, and sections per animal, which cover the entire brain area of interest. At least 4 mice per genotype were used and I need to quantify and compare soma size between 2 genotypes. it's really time consuming and endless analysis.

I heared that such analysis could be done by using Image J. Please if you know the method to do it, post me a message.
Thank you very much
Regards

[Cheuk Tam-2]

Hi Nabila,

The NeuriteTracer plugin in ImageJ and Simple NeuriteTracer in FIJI include soma measurements.  


http://fournierlab.mcgill.ca/styled-6/NeuriteTracer.html
http://fiji.sc/Simple_Neurite_Tracer

I hope this helps.


-Cheuk

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