Still Problems with nd2 Bio Format importer

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Still Problems with nd2 Bio Format importer

Greg
Hi,

so when trying to import nd2 files I see some strange grey scale image which only poorly resembles the original which is fine as validated by the NIS viewer.

Then according to this thread

http://imagej.1557.x6.nabble.com/Latest-Bio-Formats-in-Fiji-td5009834i20.html

I followed the (only 3 weeks old!) instructions from

http://trac.openmicroscopy.org/ome/ticket/12548#comment:11

Now when I try to import a nd2 file via Plugins -> Bio-Formats I get the following java exception:

(Fiji Is Just) ImageJ 2.0.0-rc-24/1.49m; Java 1.6.0_65 [64-bit]; Mac OS X 10.10.1; 21MB of 20379MB (<1%)
 
java.lang.NoClassDefFoundError: com/drew/imaging/ImageProcessingException
        at loci.formats.in.JPEGReader.<init>(JPEGReader.java:77)

I truncated the output which anyways is not fully shown by Fiji.
I repeated the instructions from the OME bug-tracker with a fresh Fiji installation twice just to make sure I did not miss any files/steps but this problem remains.

Any help appreciated !

Greets,
Greg
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Re: Still Problems with nd2 Bio Format importer

Cammer, Michael
Did you try simply resetting the LUT as Grays?
We found that importing images from our new Zyla open with an odd LUT but simply resetting the LUT and then rescaling solves the problem.
Regards,
Michael

_________________________________________
Michael Cammer, Optical Microscopy Specialist
http://ocs.med.nyu.edu/microscopy
Lab: (212) 263-3208  Cell: (914) 309-3270

________________________________________
From: ImageJ Interest Group [[hidden email]] on behalf of Greg [[hidden email]]
Sent: Wednesday, February 25, 2015 10:26 AM
To: [hidden email]
Subject: Still Problems with nd2 Bio Format importer

Hi,

so when trying to import nd2 files I see some strange grey scale image which
only poorly resembles the original which is fine as validated by the NIS
viewer.

Then according to this thread

http://imagej.1557.x6.nabble.com/Latest-Bio-Formats-in-Fiji-td5009834i20.html

I followed the (only 3 weeks old!) instructions from

http://trac.openmicroscopy.org/ome/ticket/12548#comment:11

Now when I try to import a nd2 file via Plugins -> Bio-Formats I get the
following java exception:

(Fiji Is Just) ImageJ 2.0.0-rc-24/1.49m; Java 1.6.0_65 [64-bit]; Mac OS X
10.10.1; 21MB of 20379MB (<1%)

java.lang.NoClassDefFoundError: com/drew/imaging/ImageProcessingException
        at loci.formats.in.JPEGReader.<init>(JPEGReader.java:77)

I truncated the output which anyways is not fully shown by Fiji.
I repeated the instructions from the OME bug-tracker with a fresh Fiji
installation twice just to make sure I did not miss any files/steps but this
problem remains.

Any help appreciated !

Greets,
Greg



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Re: Still Problems with nd2 Bio Format importer

Greg
Hi Michael,

omg yes indeed it was just this !!

Thank you very much.

Kind regards,
Gregor