Subtracting subsequent frames

[Vito Mennella]

Dear All,
I'm looking for a macro that would allow me to subtract subsequent frames
from a movie to highlight particle movement between frames. Does anyone have
something that might help?

All the best.

Vito
UCSF

[Anda Cornea]

Not being a good programmer, I duplicate the stack, delete the last
image from the first stack and the first image from the second stack.
You can then subtract the two stacks.

Anda

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Vito Mennella
Sent: Wednesday, June 18, 2008 2:40 PM
To: [hidden email]
Subject: Subtracting subsequent frames

Dear All,
I'm looking for a macro that would allow me to subtract subsequent
frames
from a movie to highlight particle movement between frames. Does anyone
have
something that might help?

All the best.

Vito
UCSF

[Charles M. Shaw]

I modified the AVI reader plugin to allow you to choose an interval
between frames to extract (i.e. every other frame, every third frame,
etc.) when importing an (uncompressed) AVI movie, as well as the
specific start and end frame.  Would that be of interest?

Charlie Shaw
Graduate Student
Macromolecular Science & Engineering Program
University of Michigan, Ann Arbor

On Jun 18, 2008, at 5:40 PM, Vito Mennella wrote:

> Dear All,
> I'm looking for a macro that would allow me to subtract subsequent
> frames
> from a movie to highlight particle movement between frames. Does
> anyone have
> something that might help?
>
> All the best.
>
> Vito
> UCSF
>

[Michael Schell]

 From the McMaster microscopy ImageJ plugins installed with MBF ImageJ:

Plugins-stacks-Tfunctions-deltaF up  (or down).

Mike

On Jun 18, 2008, at 5:40 PM, Vito Mennella wrote:

> Dear All,
> I'm looking for a macro that would allow me to subtract subsequent  
> frames
> from a movie to highlight particle movement between frames. Does  
> anyone have
> something that might help?
>
> All the best.
>
> Vito
> UCSF

Michael J. Schell, Ph.D., CIV, USUHS
Assist. Professor
Dept. of Pharmacology
Uniformed Services University
4301 Jones Bridge Rd.
Bethesda, MD  20814-3220
tel:  [phone]
[hidden email]
http://www.usuhs.mil/pha/mschell.html

[Arne Seitz-2]

Hi Vito,

see: http://www.embl.de/eamnet/html/kymograph.html

There you should find a plugin called StackDifference_.class which is doing
exactly what you requested.

Cheers Arne

____________________________________________________
 
Dr. Arne Seitz, Scientific Officer
Advanced Light Microscopy Facility
EMBL Heidelberg
 
[phone]

-----Ursprüngliche Nachricht-----
Von: ImageJ Interest Group [mailto:[hidden email]] Im Auftrag von Vito
Mennella
Gesendet: Mittwoch, 18. Juni 2008 23:40
An: [hidden email]
Betreff: Subtracting subsequent frames

Dear All,
I'm looking for a macro that would allow me to subtract subsequent frames
from a movie to highlight particle movement between frames. Does anyone have
something that might help?

All the best.

Vito
UCSF

[Vito Mennella]

Thanks everyone for help!




On 6/19/08 5:45 AM, "Arne seitz" <[hidden email]> wrote:

> Hi Vito,
>
> see: http://www.embl.de/eamnet/html/kymograph.html
>
> There you should find a plugin called StackDifference_.class which is doing
> exactly what you requested.
>
> Cheers Arne
>
> ____________________________________________________
>  
> Dr. Arne Seitz, Scientific Officer
> Advanced Light Microscopy Facility
> EMBL Heidelberg
>  
> [phone]
>
> -----Ursprüngliche Nachricht-----
> Von: ImageJ Interest Group [mailto:[hidden email]] Im Auftrag von Vito
> Mennella
> Gesendet: Mittwoch, 18. Juni 2008 23:40
> An: [hidden email]
> Betreff: Subtracting subsequent frames
>
> Dear All,
> I'm looking for a macro that would allow me to subtract subsequent frames
> from a movie to highlight particle movement between frames. Does anyone have
> something that might help?
>
> All the best.
>
> Vito
> UCSF