Threshold problem

[Michael Peffer]

red threshold highlighted pixels are converted to white rather than the normal black. How do I change back to normal??
I've deleted, re-imported, re-booted, no change. Cannot measure or measure particles now

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[Straub, Volko A. (Dr.)]

Hi Michael,
Have you changed the 'Dark Background' tick in the threshold dialogue? You can also invert the image after thresholding, which should solve the problem.
Hope this helps,
Volko

On 22 Jun 2019 01:34, Michael Peffer <[hidden email]> wrote:
red threshold highlighted pixels are converted to white rather than the normal black. How do I change back to normal??
I've deleted, re-imported, re-booted, no change. Cannot measure or measure particles now

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[Krs5]

Dear Micheal,


Check if under Process > Binary > Options... Black Background is selected. Some plugins or macros sometimes change this setting; it should be deselected.


Best wishes


Kees


________________________________
From: Michael Peffer <[hidden email]>
Sent: 22 June 2019 01:23
To: [hidden email]
Subject: Threshold problem

red threshold highlighted pixels are converted to white rather than the normal black. How do I change back to normal??
I've deleted, re-imported, re-booted, no change. Cannot measure or measure particles now

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[Yaman]

If you are using macro you may add this line
run("Convert to Mask");
just after color threshold and before measure.



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