Unable to work with 'Hough Circle Transform'

[ssphyss]

Hi all
I am working on imagej plugin 'Hough Circle Transform' to detect circles for
my images, however it never worked well with me every time I apply it even
though I played around with the parameters to fix it but ended up with
complete wrong results.
could you please have a look on my results and suggest something that could
fix the whole thing?
thanks
Sarah

<http://imagej.1557.x6.nabble.com/file/t381839/hepes8.png>

Hough results:
<http://imagej.1557.x6.nabble.com/file/t381839/Centroid_overlay.png>

<http://imagej.1557.x6.nabble.com/file/t381839/Hough_Transform_Series.png>



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[Gabriel Landini]

Do you *need* to use the Hough transform?
If not, the macro below seems to detect all the bubbles in your image quite
well (Result.png attached)

run("8-bit");
run("Invert");
run("Threshold Regional Gradient", "circularity=0.80 minimum=50 maximum=3600
fill_phase fill_detected method=Fast");

The Threshold Regional Gradient plugin is part of the Morphology Collection,
zip file link in this page:
http://www.mecourse.com/landinig/software/software.html

The method is described here, including a link to the paper describing it in
more detail:

http://www.mecourse.com/landinig/software/threshgrad/threshgrad.html

Hope it helps

Gabriel


On Monday, 27 November 2017 17:07:14 GMT you wrote:
> Hi all
> I am working on imagej plugin 'Hough Circle Transform' to detect circles for
> my images, however it never worked well with me every time I apply it even
> though I played around with the parameters to fix it but ended up with
> complete wrong results.
> could you please have a look on my results and suggest something that could
> fix the whole thing?
> thanks
> Sarah


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