Upside Down Peaks

[Annette E. Howard]

To Whom It May Concern:

Good evening. I'm trying to calculate the area for my protein gel bands. When I draw the rectangle and try to plot the lanes, the peaks are upside down. How do you correct it? Thank you.

Annette


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[Joel Sheffield]

Check the box in the Gel options that says "reverse lut"



Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs


On Wed, Apr 30, 2014 at 3:52 PM, Annette E. Howard <
[hidden email]> wrote:

> To Whom It May Concern:
>
> Good evening. I'm trying to calculate the area for my protein gel bands.
> When I draw the rectangle and try to plot the lanes, the peaks are upside
> down. How do you correct it? Thank you.
>
> Annette
>
>
> This communication is for use by the intended recipient and contains
> information that may be Privileged, confidential or copyrighted under
> applicable law. If you are not the intended recipient, you are hereby
> formally notified that any use, copying or distribution of this e-mail,
> in whole or in part, is strictly prohibited. Please notify the sender by
> return e-mail and delete this e-mail from your system. Unless explicitly
> and conspicuously designated as "E-Contract Intended", this e-mail does
> not constitute a contract offer, a contract amendment, or an acceptance
> of a contract offer. This e-mail does not constitute a consent to the
> use of sender's contact information for direct marketing purposes or for
> transfers of data to third parties.
>
> The dupont.com<http://dupont.com/> web address will continue in use for a
> transitional period for communications sent or received on behalf of DuPont
> Performance Coatings., which is not affiliated in any way with the DuPont
> Company.
>
> Francais Deutsch Italiano  Espanol  Portugues  Japanese  Chinese  Korean
>
>            http://www.DuPont.com/corp/email_disclaimer.html
>
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[Rasband, Wayne (NIH/NIMH) [E]]

On Apr 30, 2014, at 3:52 PM, Annette E. Howard wrote:

> To Whom It May Concern:
>
> Good evening. I'm trying to calculate the area for my protein gel bands. When I draw the rectangle and try to plot the lanes, the peaks are upside down. How do you correct it? Thank you.

Check (or uncheck if already checked) "Invert peaks" in Analyze>Gels>Gel Analyzer Options.

-wayne

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[Annette E. Howard]

Thank you very much Joel & Wayne!

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Rasband, Wayne (NIH/NIMH) [E]
Sent: Thursday, May 01, 2014 12:36 PM
To: [hidden email]
Subject: Re: Upside Down Peaks

On Apr 30, 2014, at 3:52 PM, Annette E. Howard wrote:

> To Whom It May Concern:
>
> Good evening. I'm trying to calculate the area for my protein gel bands. When I draw the rectangle and try to plot the lanes, the peaks are upside down. How do you correct it? Thank you.

Check (or uncheck if already checked) "Invert peaks" in Analyze>Gels>Gel Analyzer Options.

-wayne

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This communication is for use by the intended recipient and contains
information that may be Privileged, confidential or copyrighted under
applicable law. If you are not the intended recipient, you are hereby
formally notified that any use, copying or distribution of this e-mail,
in whole or in part, is strictly prohibited. Please notify the sender by
return e-mail and delete this e-mail from your system. Unless explicitly
and conspicuously designated as "E-Contract Intended", this e-mail does
not constitute a contract offer, a contract amendment, or an acceptance
of a contract offer. This e-mail does not constitute a consent to the
use of sender's contact information for direct marketing purposes or for
transfers of data to third parties.

The dupont.com<http://dupont.com/> web address will continue in use for a
transitional period for communications sent or received on behalf of DuPont
Performance Coatings., which is not affiliated in any way with the DuPont Company.

Francais Deutsch Italiano  Espanol  Portugues  Japanese  Chinese  Korean

           http://www.DuPont.com/corp/email_disclaimer.html

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