Using Image J to count live and dead cells in cultures

[aa299]

Hi all

I was wondering if amnybody could help me with a query regrding cell counting using image. I've stained my cultures for dead and live cells and now want to quatify them. I have tried using several methods on image J including adjusting the threshold, watershed and also downloaded a pluggin (ITCN) but for some reason i'm still not able to count the labelled cells accurately.

Any help or suggestions in regards to this will be greatly appreciated.
Many Thanks

Asha

[pmbcellcounter]

*bump*

Surprise! I am facing the exact similar situation in 2013!

Can anyone help? Thanks in advance...

[Shall, Sydney]

I would be inclined to use a double staining technique; perhaps and also
a non-vital dye with a different spectrum.
Then count the number of cells with each colour.
Sydney



On 05/02/2013 04:44, pmbcellcounter wrote:

> *bump*
>
> Surprise! I am facing the exact similar situation in 2013!
>
> Can anyone help? Thanks in advance...
>
>
>
> --
> View this message in context: http://imagej.1557.n6.nabble.com/Using-Image-J-to-count-live-and-dead-cells-in-cultures-tp3686899p5001666.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

... [show rest of quote]

--
Professor Sydney Shall,
Department of Haematological Medicine,
King's College London,
Medical School,
123 Coldharbour Lane,
LONDON SE5 9NU,
Tel & Fax: [phone],
E-Mail: sydney.shall,
[correspondents outside the College should add; @kcl.ac.uk]
www.kcl.ac.uk

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