Western Blot

[Veronica Dy]

Dear ImageJ users,

I have a quick question.

Am I able to use ImageJ to analyse the bands I have from Western Blot
analyses?  If so, what is the best plugin to use?

Thank you very much for your time.

Regards,
Veronica.

[diah taman]

Dear Veronica,
you can use it. Its very easy to do, like below:
1.open your file (western blott do you have),
2. klik image/color/RGB split
3. chose one of figure
4. use rectangular to select "band" do you need for analyze
5. klik ctrl+1
6. klik analyze/plot profile
you find result in grey scale. If you want to change scale to "OD". you should make "optical density caliberation.
open http://rsb.info.nih.gov/ij/docs/examples/calibration/index.html
 
Have a nice day,
 
Best wishes,
 
Al Munawir, MD
Toxicology Molecular Lab.
Gyeong Sang National University
900 Gajwa-Dong, Jinju 660-701
South of Korea


--- On Tue, 6/24/08, Veronica Dy <[hidden email]> wrote:

From: Veronica Dy <[hidden email]>
Subject: Western Blot
To: [hidden email]
Date: Tuesday, June 24, 2008, 12:54 PM

Dear ImageJ users,

I have a quick question.

Am I able to use ImageJ to analyse the bands I have from Western Blot
analyses?  If so, what is the best plugin to use?

Thank you very much for your time.

Regards,
Veronica.

[Veronica Dy]

Thank you for all the feedback.
Mostly appreciated.

All the best to everyone!

Veronica.





On 24/6/08 6:00 PM, "Colin Rickman" <[hidden email]> wrote:

> No plug-in required. It's built in to ImageJ. Instructions are here
> (http://rsb.info.nih.gov/ij/docs/menus/analyze.html#gels) although make
> sure if you are measuring the bands from film that they aren't saturated
> and that you scan in the image from the film using transmitted light
> (not reflective).
>
> Colin