ImageJ

anisotropy image

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Virginia Borroni

anisotropy image

Hi,
I would like to know if Imagej can be used to generate an anisotropy image.
I have tryed but when I do the math with the images in both channels
(paralel and perpendicular) I only get a black image in wich every pixel is
0. I know that is because I get negative and fractional numbers, but how can
I overcome this problem? Thanks a lot
Virginia Borroni

Michael Doube

Re: anisotropy image

Hi Virginia

What do you mean by "anisotropy image"?

There are a few ways to calculate anisotropy, including star volume,
mean intercept length and autocorrelation. Is this what you want to do?

Mike

Virginia Borroni wrote:
> Hi,
> I would like to know if Imagej can be used to generate an anisotropy image.
> I have tryed but when I do the math with the images in both channels
> (paralel and perpendicular) I only get a black image in wich every pixel is
> 0. I know that is because I get negative and fractional numbers, but how can
> I overcome this problem? Thanks a lot
> Virginia Borroni

Virginia Borroni

Re: anisotropy image

We do it by homoFRET on cells.
Quoting Michael Doube <[hidden email]>:

> Hi Virginia
>
> What do you mean by "anisotropy image"?
>
> There are a few ways to calculate anisotropy, including star volume,
> mean intercept length and autocorrelation. Is this what you want to do?
>
> Mike
>
> Virginia Borroni wrote:
>> Hi,
>> I would like to know if Imagej can be used to generate an anisotropy image.
>> I have tryed but when I do the math with the images in both channels
>> (paralel and perpendicular) I only get a black image in wich every pixel is
>> 0. I know that is because I get negative and fractional numbers, but how can
>> I overcome this problem? Thanks a lot
>> Virginia Borroni

Maria Virginia Borroni
INIBIBB
C La Carrindanga Km 7 CC857. B8000FWB.
Bahia Blanca. Buenos Aires. Argentina.
Tel.#: 54-291-4861201. interno 127
Fax#: 54-291-4861200
[hidden email]

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Michael Elbaum

Re: anisotropy image

In reply to this post by Virginia Borroni

Hi. You should convert the images to 32 bit format, which holds fractional values.
Michael

>>> Virginia Borroni <[hidden email]> 05/15/09 9:51 PM >>>
We do it by homoFRET on cells.
Quoting Michael Doube <[hidden email]>:

Virginia Borroni

Re: anisotropy image

Thanks a lot, I will try that!
Virginia

Quoting Michael Elbaum <[hidden email]>:

> Hi. You should convert the images to 32 bit format, which holds
> fractional values.
> Michael
>
>
>>>> Virginia Borroni <[hidden email]> 05/15/09 9:51 PM >>>
> We do it by homoFRET on cells.
> Quoting Michael Doube <[hidden email]>:
>
>> Hi Virginia
>>
>> What do you mean by "anisotropy image"?
>>
>> There are a few ways to calculate anisotropy, including star volume,
>> mean intercept length and autocorrelation. Is this what you want to do?
>>
>> Mike
>>
>> Virginia Borroni wrote:
>>> Hi,
>>> I would like to know if Imagej can be used to generate an anisotropy image.
>>> I have tryed but when I do the math with the images in both channels
>>> (paralel and perpendicular) I only get a black image in wich every pixel is
>>> 0. I know that is because I get negative and fractional numbers,
>>> but how can
>>> I overcome this problem? Thanks a lot
>>> Virginia Borroni
>
>
>
> Maria Virginia Borroni
> INIBIBB
> C La Carrindanga Km 7 CC857. B8000FWB.
> Bahia Blanca. Buenos Aires. Argentina.
> Tel.#: 54-291-4861201. interno 127
> Fax#: 54-291-4861200
> [hidden email]
>
>
> ----------------------------------------------------------------
> This message was sent using IMP, the Internet Messaging Program.
>