area measurment

[thuraya]

Hello,
please help me ,, i need to measure the blue color to the whole imag

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[hesham977]

Excellent question, we need to learn it step by step please


On Fri, Oct 11, 2013 at 11:00 PM, Thuraya Medhat
<[hidden email]>wrote:

> Hello,
> please help me ,, i need to measure the blue color to the whole imag
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>



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*Dr. Hesham Noaman Abdelraheem Mustafa
Assistant Professor,
Faculty of Medicine, King Abdulaziz University,
e-Library Consultant,
Student Support Unit Member,
Nutrition & Metabolism Module Coordinator.
Address: P.O.Box 80205
               Jeddah, 21589
               Saudi Arabia.
Phone:
           (Office):  [phone] Ext. 22016
           (Mobile): [phone]
           (Egypt):  [phone]*

**

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[ctrueden]

Hi all,

> i need to measure the blue color to the whole imag
> Excellent question, we need to learn it step by step please

1) Press Shift+T to open Threshold Color.
2) Adjust Hue sliders to encompass desired colors in the range of what you
consider "blue" here.
3) Adjust Saturation and Brightness sliders to full 0-255 range.
4) Click Select button to create a selection of the blue areas.
5) Click your image window to reactive it.
6) Press M to measure statistics including area (which in IJ1 terms is a
count of pixels).

If you want to repeat the analysis you will want to run Plugins > Macros >
Record first and then click the Macro button after setting the Hue to
generate an appropriate macro.

-Curtis


On Fri, Oct 11, 2013 at 3:06 PM, Dr.Hesham Noaman AbdelRaheem Mustafa <
[hidden email]> wrote:

> Excellent question, we need to learn it step by step please
>
>
> On Fri, Oct 11, 2013 at 11:00 PM, Thuraya Medhat
> <[hidden email]>wrote:
>
> > Hello,
> > please help me ,, i need to measure the blue color to the whole imag
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
>
>
> --
>
> *Dr. Hesham Noaman Abdelraheem Mustafa
> Assistant Professor,
> Faculty of Medicine, King Abdulaziz University,
> e-Library Consultant,
> Student Support Unit Member,
> Nutrition & Metabolism Module Coordinator.
> Address: P.O.Box 80205
>                Jeddah, 21589
>                Saudi Arabia.
> Phone:
>            (Office):  [phone] Ext. 22016
>            (Mobile): [phone]
>            (Egypt):  [phone]*
>
> **
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[thuraya]

i tried this already, the problem is that i can't accurately do it for the
blue color only
i still have other colors in the image


On Fri, Oct 11, 2013 at 10:31 PM, Curtis Rueden <[hidden email]> wrote:

> Hi all,
>
> > i need to measure the blue color to the whole imag
> > Excellent question, we need to learn it step by step please
>
> 1) Press Shift+T to open Threshold Color.
> 2) Adjust Hue sliders to encompass desired colors in the range of what you
> consider "blue" here.
> 3) Adjust Saturation and Brightness sliders to full 0-255 range.
> 4) Click Select button to create a selection of the blue areas.
> 5) Click your image window to reactive it.
> 6) Press M to measure statistics including area (which in IJ1 terms is a
> count of pixels).
>
> If you want to repeat the analysis you will want to run Plugins > Macros >
> Record first and then click the Macro button after setting the Hue to
> generate an appropriate macro.
>
> -Curtis
>
>
> On Fri, Oct 11, 2013 at 3:06 PM, Dr.Hesham Noaman AbdelRaheem Mustafa <
> [hidden email]> wrote:
>
> > Excellent question, we need to learn it step by step please
> >
> >
> > On Fri, Oct 11, 2013 at 11:00 PM, Thuraya Medhat
> > <[hidden email]>wrote:
> >
> > > Hello,
> > > please help me ,, i need to measure the blue color to the whole imag
> > >
> > > --
> > > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> > >
> >
> >
> >
> > --
> >
> > *Dr. Hesham Noaman Abdelraheem Mustafa
> > Assistant Professor,
> > Faculty of Medicine, King Abdulaziz University,
> > e-Library Consultant,
> > Student Support Unit Member,
> > Nutrition & Metabolism Module Coordinator.
> > Address: P.O.Box 80205
> >                Jeddah, 21589
> >                Saudi Arabia.
> > Phone:
> >            (Office):  [phone] Ext. 22016
> >            (Mobile): [phone]
> >            (Egypt):  [phone]*
> >
> > **
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[Jacqueline Ross]

Hi Thuraya,

You could try Gabriel Landini's Colour Deconvolution plugin http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html  instead. I just tried it on your image using the H&E vectors and it worked quite well to separate out the pink and blue.

Your image looks a bit weird to me but perhaps that's due to the compression.

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Thuraya Medhat
Sent: Saturday, 12 October 2013 11:37 a.m.
To: [hidden email]
Subject: Re: area measurment

i tried this already, the problem is that i can't accurately do it for the blue color only i still have other colors in the image


On Fri, Oct 11, 2013 at 10:31 PM, Curtis Rueden <[hidden email]> wrote:

> Hi all,
>
> > i need to measure the blue color to the whole imag Excellent
> > question, we need to learn it step by step please
>
> 1) Press Shift+T to open Threshold Color.
> 2) Adjust Hue sliders to encompass desired colors in the range of what
> you consider "blue" here.
> 3) Adjust Saturation and Brightness sliders to full 0-255 range.
> 4) Click Select button to create a selection of the blue areas.
> 5) Click your image window to reactive it.
> 6) Press M to measure statistics including area (which in IJ1 terms is
> a count of pixels).
>
> If you want to repeat the analysis you will want to run Plugins >
> Macros > Record first and then click the Macro button after setting
> the Hue to generate an appropriate macro.
>
> -Curtis
>
>
> On Fri, Oct 11, 2013 at 3:06 PM, Dr.Hesham Noaman AbdelRaheem Mustafa
> < [hidden email]> wrote:
>
> > Excellent question, we need to learn it step by step please
> >
> >
> > On Fri, Oct 11, 2013 at 11:00 PM, Thuraya Medhat
> > <[hidden email]>wrote:
> >
> > > Hello,
> > > please help me ,, i need to measure the blue color to the whole
> > > imag
> > >
> > > --
> > > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> > >
> >
> >
> >
> > --
> >
> > *Dr. Hesham Noaman Abdelraheem Mustafa Assistant Professor, Faculty
> > of Medicine, King Abdulaziz University, e-Library Consultant,
> > Student Support Unit Member, Nutrition & Metabolism Module
> > Coordinator.
> > Address: P.O.Box 80205
> >                Jeddah, 21589
> >                Saudi Arabia.
> > Phone:
> >            (Office):  [phone] Ext. 22016
> >            (Mobile): [phone]
> >            (Egypt):  [phone]*
> >
> > **
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[Alistair]

You say the blue is difficult to PIC out accros the whole image. Looking at it there seems to be a colour gradient which may confuse the software and its tolerances. I would recommend trying 'subtract background' to level it out first