automated nematode count

[Eva Brusselman]

Dear all,
 
I would like to count the % of living nematodes in a stack of 5 pictures
(taken every 5 sec). Manually I can do this measurement with the
roiManager, but I did not succeed to do this automatically. I tried
MTrack2, but there are some nematodes who are not recognized by this
pugin. Moreover nematodes that overlap on all the pictures are not
recognized as separate nematodes.
 
Besides MTrack2, is there another plugin I can use to do this
measurement?
 
Another question: is there a plugin that I can use to make a difference
between nematodes and debris on the basis of shape?
 
Thanks for your help.
 
Eva
 
ir. Eva Brusselman
[hidden email]
Instituut voor Landbouw- en Visserijonderzoek (ILVO)
Wetenschappelijke Instelling van de Vlaamse Gemeenschap
Eenheid: Technologie en Voeding
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