automated t- or z-plot in an image- stack using a variable rectangular roi

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automated t- or z-plot in an image- stack using a variable rectangular roi

Gensch, Thomas
dear imagej community,

i am posing a question, that might be trivial. i want to analyse stacks of
fluorescence microscopy images as a function of time (50-500 time points).
the images are pretty diffuse and the points of interest are only 2*2, 3*3
or 4*4 pixels. only small parts in the image will react. i would like to
have an automated routine that moves a 2by, 3by3 or 4by4  mask over the
image, determines the time course at any point and store the fluo_intensity
vs. time curves in a 3-d array.

is there any such plugin available?? or an advice, how to produce it
without too much programming effort.


best regards

thomas gensch
Dr. Thomas Gensch
Institute of Neuroscience and Biophysics 1 (INB-1)
Research Centre Juelich
D-52425 Germany
Tel: +49-2461-618068
Fax: +49-2461-614216
email: [hidden email]

http://www.fz-juelich.de/inb/inb-1/
http://www.fz-juelich.de/inb/inb-1/gensch_t/



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