Hi,
When I try to use 3D filtering on a stack that comes from a composite
stack that has just been splitted with Color/split Channel, the 3D
filters do not work and indicate a bitDepth=0. When I manually change to
16 bits it's working.
run("Confocal Series (2.2MB)");
run("Split Channels");
selectWindow("C1-confocal-series.tif");
run("Mean 3D...", "x=2 y=2 z=2"); // DOES NOT WORK
run("16-bit");
run("8-bit");
run("Mean 3D...", "x=2 y=2 z=2"); // WORKS
Thomas
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Thomas Boudier, MCU Université Pierre et Marie Curie,
Modélisation Cellulaire et Imagerie Biologique (EE1),
IFR 83, Bat B 7ème étage, porte 723, Campus Jussieu.
Tel : 01 44 27 46 92 Fax : 01 44 27 22 91
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