calibrate time-series image photobleaching in ImageJ

[Liu, Dongfang (NIH/NIAID) [F]]

Hi All,

I have time-series image which tracks the intensity as a function of time. There is photobleaching in my time-series image stack. Because I tracked the intensity of non-signal region, there is obvious decay of intensity. I think such photobleaching must affect the intensity of signal region. Do you know how to correct photobleaching in ImageJ? In other words, how to avoid my signals are affected by photobleaching? How to calibrate it?

Thanks.
Don

[lechristophe]

Have a look here :
http://www.macbiophotonics.ca/imagej/t.htm#t_bleach

In the MBF distribution of ImageJ, there is a plugin that is using an
exponentional decay to model bleach. There is also a macro from Jens
Rietdorf, however the link seems to be broken :

http://www.embl-heidelberg.de/ExternalInfo/almf/htdocs/almf_website/html/downloads.html

Christophe

On Mon, Jun 1, 2009 at 8:07 AM, Liu, Dongfang (NIH/NIAID) [F] <
[hidden email]> wrote:

> Hi All,
>
> I have time-series image which tracks the intensity as a function of time.
> There is photobleaching in my time-series image stack. Because I tracked the
> intensity of non-signal region, there is obvious decay of intensity. I think
> such photobleaching must affect the intensity of signal region. Do you know
> how to correct photobleaching in ImageJ? In other words, how to avoid my
> signals are affected by photobleaching? How to calibrate it?
>
> Thanks.
> Don
>

[Liu, Dongfang (NIH/NIAID) [F]]

Hi Christophe,

Thank you for your kind reply.
Could you please send me the plugin called 'T-function'? I cannot find it in the ImageJ website.

Thank you very much!

Best regards
Don

-----Original Message-----
From: Christophe Leterrier [mailto:[hidden email]]
Sent: Monday, June 01, 2009 2:55 AM
To: List IMAGEJ
Subject: Re: calibrate time-series image photobleaching in ImageJ

Have a look here :
http://www.macbiophotonics.ca/imagej/t.htm#t_bleach

In the MBF distribution of ImageJ, there is a plugin that is using an
exponentional decay to model bleach. There is also a macro from Jens
Rietdorf, however the link seems to be broken :

http://www.embl-heidelberg.de/ExternalInfo/almf/htdocs/almf_website/html/downloads.html

Christophe

On Mon, Jun 1, 2009 at 8:07 AM, Liu, Dongfang (NIH/NIAID) [F] <
[hidden email]> wrote:

> Hi All,
>
> I have time-series image which tracks the intensity as a function of time.
> There is photobleaching in my time-series image stack. Because I tracked the
> intensity of non-signal region, there is obvious decay of intensity. I think
> such photobleaching must affect the intensity of signal region. Do you know
> how to correct photobleaching in ImageJ? In other words, how to avoid my
> signals are affected by photobleaching? How to calibrate it?
>
> Thanks.
> Don
>