For years we have been using the Zeiss 710 for timelapse experiments. When we open the files using the BioFormats importer in ImageJ the metadata reports a start time for the experiments and timestamps for each timepoint.
Last night I did a timelapse experiment with a new Zeiss 700 and when I opened the files there were no metadata for the experiment time nor timestamps. There is, however, a tag that says Information|Image|T|BinaryList|AttachmentName| #1 TimeStamps Does anyone know how we can get the start time and timestamps info? We need both. Thank you! =========================================================================== Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center Cell: 914-309-3270 Lab: 212-263-3208 http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/ ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
Hi Michael,
> when I opened the files there were no metadata for the experiment time > nor timestamps. Sounds like an issue with Bio-Formats. Have you considered reporting the bug to the OME team [1] and sending them a sample image [2]? Regards, Curtis [1] https://www.openmicroscopy.org/site/support/bio-formats5/about/bug-reporting.html [2] http://qa.openmicroscopy.org.uk/qa/upload/ On Wed, Jun 25, 2014 at 9:05 AM, Cammer, Michael <[hidden email] > wrote: > For years we have been using the Zeiss 710 for timelapse experiments. > When we open the files using the BioFormats importer in ImageJ the > metadata reports a start time for the experiments and timestamps for each > timepoint. > > Last night I did a timelapse experiment with a new Zeiss 700 and when I > opened the files there were no metadata for the experiment time nor > timestamps. There is, however, a tag that says > Information|Image|T|BinaryList|AttachmentName| #1 TimeStamps > > Does anyone know how we can get the start time and timestamps info? We > need both. > > Thank you! > > =========================================================================== > Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU > Langone Medical Center > Cell: 914-309-3270 Lab: 212-263-3208 > http://ocs.med.nyu.edu/microscopy & > http://www.med.nyu.edu/skirball-lab/dustinlab/ > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of the > intended recipient(s) and may contain information that is proprietary, > confidential, and exempt from disclosure under applicable law. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > have received this email in error please notify the sender by return email > and delete the original message. Please note, the recipient should check > this email and any attachments for the presence of viruses. The > organization accepts no liability for any damage caused by any virus > transmitted by this email. > ================================= > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html > -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
Thanks for the replies both on & off list.
The simplest way to solve this problem is to use Save As and choose the LSM format since this works fine with the LOCI plugins for importing into ImageJ. Regards, Michael =========================================================================== Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center Cell: 914-309-3270 Lab: 212-263-3208 http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/ ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
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