cell counting problem

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cell counting problem

jay chen
Dear imagej experts:

      I want to count cells from an image (http://www.badongo.com/pic/4652704).
The procedures I have followed are  WCIF online manual and Visual
Guide IV: Measuring cells with ImageJ
(http://naranja.umh.es/~atg/tutorials/VGIV-MeasuringCellsImageJ.pdf).
Unfortunately, some cells can not be seperated.  Can anyone provide
some hints?  Thanks for hlep.

                              Best Regards

                              Jay Chen
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Re: cell counting problem

Tony Collins-4
Hi Jay,

Try inverting the image (Image>Invert) then set a threshold (Image>Adjust>Threshold) then use the find maxima command (Process>Binary>Find Maxima).

The illumination is not very even - you may want to check that the microscope condenser is set up properly. This may mean you'll have trouble setting the threshold. You could try a background subtraction with a small radius - Image>Subtract Background: light background; radius 10 (which is usually too small).

If you've a true-color image of this you may also try to use the colour-segmentations plugins.

Hope this helps.

BTW, a few years ago the WCIF_ImageJ manual and collection migrated to MBF_ImageJ:
http://www.macbiophotonics.ca/imagej/
http://www.macbiophotonics.ca/downloads.htm

Regards,
Tony

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of jay chen
Sent: October-28-08 9:28 PM
To: [hidden email]
Subject: cell counting problem

Dear imagej experts:

      I want to count cells from an image (http://www.badongo.com/pic/4652704).
The procedures I have followed are  WCIF online manual and Visual
Guide IV: Measuring cells with ImageJ
(http://naranja.umh.es/~atg/tutorials/VGIV-MeasuringCellsImageJ.pdf).
Unfortunately, some cells can not be seperated.  Can anyone provide
some hints?  Thanks for hlep.

                              Best Regards

                              Jay Chen